Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   WOC43_RS10485 Genome accession   NZ_CP150749
Coordinates   2109123..2109545 (+) Length   140 a.a.
NCBI ID   WP_258922346.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22699     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2104123..2114545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC43_RS10445 (WOC43_10445) - 2104167..2105153 (+) 987 WP_000161314.1 ROK family glucokinase -
  WOC43_RS10450 (WOC43_10450) - 2105153..2105482 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WOC43_RS10455 (WOC43_10455) - 2105479..2106102 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  WOC43_RS10460 (WOC43_10460) comGA 2106154..2107128 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  WOC43_RS10465 (WOC43_10465) comGB 2107100..2108170 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  WOC43_RS10470 (WOC43_10470) comGC 2108184..2108495 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WOC43_RS10475 (WOC43_10475) comGD 2108473..2108919 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  WOC43_RS10480 (WOC43_10480) comGE 2108906..2109205 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  WOC43_RS10485 (WOC43_10485) comGF 2109123..2109545 (+) 423 WP_258922346.1 competence type IV pilus minor pilin ComGF Machinery gene
  WOC43_RS10490 (WOC43_10490) - 2109623..2110795 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  WOC43_RS10495 (WOC43_10495) - 2110848..2110952 (+) 105 Protein_2020 type IV pilus minor pilin ComGF family protein -
  WOC43_RS10500 (WOC43_10500) - 2111049..2111195 (+) 147 WP_001789879.1 hypothetical protein -
  WOC43_RS10505 (WOC43_10505) - 2111185..2111709 (+) 525 WP_001015117.1 shikimate kinase -
  WOC43_RS10510 (WOC43_10510) gcvT 2111868..2112959 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WOC43_RS10515 (WOC43_10515) gcvPA 2112979..2114325 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 16413.66 Da        Isoelectric Point: 10.0943

>NTDB_id=878431 WOC43_RS10485 WP_258922346.1 2109123..2109545(+) (comGF) [Staphylococcus aureus strain TUM22699]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVDKVRIIV

Nucleotide


Download         Length: 423 bp        

>NTDB_id=878431 WOC43_RS10485 WP_258922346.1 2109123..2109545(+) (comGF) [Staphylococcus aureus strain TUM22699]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTG
ATAAAGTCCGTATAATTGTGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(66-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

95

0.95
  comGF Staphylococcus aureus N315

96.241

95

0.914