Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RGB74_RS19490 Genome accession   NZ_CP134049
Coordinates   3815551..3816924 (-) Length   457 a.a.
NCBI ID   WP_310760869.1    Uniprot ID   -
Organism   Bacillus sp. NEB1478     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3810551..3821924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB74_RS19470 (RGB74_19470) ispF 3812121..3812594 (-) 474 WP_310760865.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  RGB74_RS19475 (RGB74_19475) ispD 3812591..3813286 (-) 696 WP_310760866.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RGB74_RS19480 (RGB74_19480) - 3813306..3814397 (-) 1092 WP_310760867.1 PIN/TRAM domain-containing protein -
  RGB74_RS19485 (RGB74_19485) disA 3814472..3815545 (-) 1074 WP_310760868.1 DNA integrity scanning diadenylate cyclase DisA -
  RGB74_RS19490 (RGB74_19490) radA 3815551..3816924 (-) 1374 WP_310760869.1 DNA repair protein RadA Machinery gene
  RGB74_RS19495 (RGB74_19495) clpC 3817153..3819573 (-) 2421 WP_310760870.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  RGB74_RS19500 (RGB74_19500) - 3819588..3820658 (-) 1071 WP_310760871.1 protein arginine kinase -
  RGB74_RS19505 (RGB74_19505) - 3820662..3821195 (-) 534 WP_310760872.1 UvrB/UvrC motif-containing protein -
  RGB74_RS19510 (RGB74_19510) - 3821232..3821699 (-) 468 WP_310760873.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49750.35 Da        Isoelectric Point: 6.6166

>NTDB_id=878389 RGB74_RS19490 WP_310760869.1 3815551..3816924(-) (radA) [Bacillus sp. NEB1478]
MAKVKSIFLCQDCGYESPKWMGKCPGCQAWNTMVEETIRPEKSVRGLSSGNSAIKQKPQSIVEVKSEQEPRIPTNYQEFN
RVLGGGIVPGSLVLVGGDPGIGKSTLLLQTSAQLAQKGERVLYISGEESVKQTKLRADRLDIAAKDLYVLAETDMELIEQ
AMDDIRPSVMIIDSIQTVYRSEVTSAPGSVSQVRECTSHLMRMAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDAVLYFE
GERHHTFRILRAVKNRFGSTNEIGVFEMKEEGLDEVLNPSEIFLEERSKGAAGSTVVASLEGTRPMLVELQALISPTSFG
NPRRMATGIEHNRVSLLMAVLEKRVGLLLQNQDAYLNVAGGVRLDEPAIDLAIAVSIASSFRDVPTKPTDVMIGEIGLTG
EVRRVSRIDQRVHEAAKLGFTRAIIPEKNIGGWTVPKGIEVLGVSTVYEALQAALGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=878389 RGB74_RS19490 WP_310760869.1 3815551..3816924(-) (radA) [Bacillus sp. NEB1478]
ATGGCGAAAGTAAAATCAATCTTTCTTTGCCAGGATTGCGGTTATGAATCTCCTAAATGGATGGGGAAATGTCCAGGATG
TCAGGCGTGGAATACGATGGTCGAGGAAACGATCCGTCCTGAAAAAAGTGTTAGAGGATTAAGTTCCGGCAATTCAGCAA
TCAAGCAAAAGCCACAATCCATAGTGGAAGTGAAAAGTGAACAGGAACCTCGGATTCCAACAAACTATCAAGAGTTCAAC
CGGGTGCTAGGCGGCGGAATCGTTCCAGGTTCTTTAGTTTTAGTTGGCGGCGATCCCGGGATAGGGAAATCAACGCTTCT
TCTGCAAACTTCAGCTCAGCTCGCTCAAAAAGGAGAACGCGTTCTCTACATATCAGGTGAGGAATCAGTTAAGCAAACGA
AGTTAAGAGCAGACAGACTTGATATAGCAGCAAAAGATTTATATGTATTGGCAGAAACAGATATGGAGCTGATCGAGCAG
GCGATGGATGATATTAGGCCATCTGTCATGATTATTGACTCCATTCAAACGGTTTATCGTTCAGAAGTAACTTCAGCTCC
TGGCAGTGTTTCCCAAGTAAGAGAATGTACATCACATCTTATGCGGATGGCAAAGACGAAAGGTATTGCTATTTTTATAG
TCGGACATGTTACTAAAGAAGGAGCGATTGCAGGTCCTCGCCTTTTAGAGCACATGGTGGATGCTGTTTTATATTTTGAA
GGGGAACGTCATCATACTTTTCGTATTTTAAGGGCTGTTAAAAACCGATTCGGGTCAACGAATGAAATCGGTGTATTTGA
AATGAAAGAAGAAGGACTTGATGAAGTTTTAAATCCTTCTGAAATTTTTTTAGAAGAACGATCAAAAGGAGCAGCGGGAT
CTACCGTTGTGGCATCATTAGAAGGCACACGCCCAATGCTTGTCGAACTTCAGGCACTTATCTCCCCTACCAGCTTCGGA
AACCCTAGAAGAATGGCAACAGGTATTGAACATAACCGAGTTTCACTATTGATGGCAGTTCTGGAGAAAAGGGTAGGCTT
ACTTCTTCAAAATCAGGATGCTTATTTGAATGTGGCAGGTGGAGTAAGACTTGATGAACCGGCTATAGACTTAGCGATAG
CAGTGAGCATTGCATCGAGTTTCAGAGATGTTCCGACAAAACCAACAGATGTTATGATCGGAGAAATCGGTCTAACAGGA
GAAGTTAGAAGAGTCTCACGTATTGATCAAAGGGTACATGAGGCAGCAAAGTTAGGATTTACGCGTGCTATTATACCTGA
AAAAAATATAGGTGGATGGACTGTGCCAAAGGGAATTGAAGTTCTCGGCGTATCCACTGTTTATGAGGCATTACAAGCAG
CGTTAGGAGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(349-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.891

100

0.751
  radA Streptococcus pneumoniae Rx1

62.252

99.125

0.617
  radA Streptococcus pneumoniae D39

62.252

99.125

0.617
  radA Streptococcus pneumoniae R6

62.252

99.125

0.617
  radA Streptococcus pneumoniae TIGR4

62.252

99.125

0.617
  radA Streptococcus mitis NCTC 12261

61.842

99.781

0.617
  radA Streptococcus mitis SK321

62.031

99.125

0.615