Detailed information NTG0878382

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RGB74_RS12215
• Genome accession: NZ_CP134049
• Coordinates: 2470766..2474512 (-)
• Length: 1248 a.a.
• NCBI ID: WP_310759578.1
• Uniprot ID: -
• Organism: Bacillus sp. NEB1478
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2465766..2479512

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGB74_RS12205 (RGB74_12205)	-	2466176..2469577 (-)	3402	WP_310759577.1	SMC family ATPase	-
RGB74_RS12210 (RGB74_12210)	-	2469574..2470754 (-)	1181	Protein_2425	exonuclease SbcCD subunit D	-
RGB74_RS12215 (RGB74_12215)	addA	2470766..2474512 (-)	3747	WP_310759578.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RGB74_RS12220 (RGB74_12220)	addB	2474509..2478012 (-)	3504	WP_310759579.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RGB74_RS12225 (RGB74_12225)	-	2478291..2479349 (-)	1059	WP_310759580.1	GGDEF domain-containing protein	-

Sequence

Protein

• Length: 1248 a.a.
• Molecular weight: 142880.57 Da
• Isoelectric Point: 4.8773

>NTDB_id=878382 RGB74_RS12215 WP_310759578.1 2470766..2474512(-) (addA) [Bacillus sp. NEB1478]
MKTLIPKPEGSTWTDEQWQAIQARGENILVAAAAGSGKTAVLVERIITMIREQSADVDRLLIVTFTNAAAAEMRKRIGEA
IDKELSLHPSSLHLRRQLNLLNKASISTLHSFCIEVLRKHYYETDLDPGFRVSEETEAELIRQEVIEDLFEEEYSKGEDH
AFYQVVDAYSSDRNDIDLQKLVLSLYDFARSHPDPESWLDQMAQNYNVSHLSIDDLPWTKELLLDIGLQIEGLKAMTIRA
NELAESPGGPAAYVSALQEDLQFLNELNQAKNSWNSLYEIFQNFEPAKLKAVRGQEVDEILKEKVKGMRDTIKKRLSTMK
EEYFERHPESYVTHIEEMAPIVLELTSLVKEFGRRYSQAKREKSLVDFGDLEHYCLHVLKGEGSTMDHPVPSDAANEYRE
RFVEVLVDEYQDTNFVQESIVRLVAKDEDQGNMFMVGDVKQSIYRFRLAEPLLFLSKYKQFTKEGGAGLRIDLAKNFRSR
ESVLHATNYLFRQIMNETVGEIEYSEDAELKLGASYYPEFENNEPELILINKSEPAVDEEGLDTGEMEKSELETVQLEAR
VIAEKIKEIIGANGEPPHLVFDKYTKSMRPAKYKDIVMLFRATSAWAPVILEEFKQQGIPAYAELSTGYFDATEIHVMMS
LLKVIDNPLQDIPLASVLRSPLVGMSGEELAIVRLIQKKDNYLGAAEQYSKSGSDPAIREKVSFFLEKLDTWRNQARQGS
LADLIWDIYRETGYFDYVGGLPGGKQRQANLRALYDRAKMYESTSFRGLFRFLRFIERMQERGKDLGAARALGEQEDVVR
IMTIHKSKGLEFPIVFVGGLNKQFNTRDLKEKVLLHKELGLGTQYINPQYRVMYPTLPKLAITGRKKMELIAEEMRVLYV
ALTRAKEKLYLIGTVNDIEKSADKWSDSLYEESWLLPDFERTQVMSYLDWIGPAVIRHKNAAPILAHLQSSEQGRGEVFI
DQADWKITILPASDYEELNIEQKQDQQELITTLQKGETVGLTSEANEKIIQRLEWRYEYSQSSVTRSKQSVTEIKKQFQS
HDEYSGTDLVKVAQPVIHERPRFMQEAQLNAAEAGTAMHMVMQHIDFNSSISLEDLEEQLAAMTAKELLTEEQARYIQLP
QIKAFIEGPLGRRIIEIGNIHREIPFSLALPLSETKTGWSGEEDEHILVQGVIDCLVEEDDGFILVDYKTDKITNRFTNG
FIGAKQVLLDRYEVQLTLYAKAIERILKKPVKESYLYFFDGGHILQVK

Nucleotide

• Length: 3747 bp

>NTDB_id=878382 RGB74_RS12215 WP_310759578.1 2470766..2474512(-) (addA) [Bacillus sp. NEB1478]
TTGAAAACGTTAATTCCTAAACCTGAAGGTTCTACCTGGACAGATGAACAATGGCAGGCGATTCAAGCAAGAGGTGAAAA
TATTCTCGTAGCTGCTGCCGCTGGATCCGGAAAGACGGCTGTCCTTGTTGAACGAATAATTACGATGATTCGGGAACAAT
CTGCTGATGTTGACAGACTGCTTATCGTAACGTTTACAAATGCAGCAGCTGCAGAAATGAGAAAGAGAATTGGTGAAGCG
ATCGATAAAGAACTGTCACTGCATCCTTCTTCGCTGCATTTGAGAAGACAGCTGAATTTACTCAATAAAGCTTCAATCTC
AACTCTCCATTCGTTCTGTATTGAAGTACTTCGAAAACATTATTACGAAACGGATCTGGACCCCGGTTTTCGTGTGTCAG
AGGAAACAGAAGCAGAACTGATTCGCCAAGAAGTGATCGAGGATTTGTTTGAAGAAGAATACAGCAAGGGGGAAGATCAC
GCTTTTTATCAAGTAGTGGACGCCTATAGTTCCGACCGAAATGACATCGATCTGCAAAAGCTTGTGCTGTCACTCTATGA
TTTTGCAAGAAGCCACCCAGATCCTGAGAGCTGGCTTGATCAAATGGCTCAAAACTATAATGTGAGTCATTTATCAATCG
ATGATCTGCCTTGGACAAAAGAGCTTCTCTTGGACATTGGATTGCAGATTGAAGGTCTTAAAGCAATGACAATCCGTGCA
AATGAGCTTGCCGAATCCCCAGGTGGTCCGGCGGCATATGTTTCTGCGTTACAAGAAGACTTGCAGTTCCTGAACGAATT
AAATCAGGCTAAGAACAGCTGGAATTCTTTATACGAAATCTTTCAAAACTTTGAACCAGCAAAGTTAAAAGCAGTGCGAG
GACAAGAAGTTGATGAAATTCTAAAAGAAAAAGTAAAAGGTATGAGGGATACCATTAAGAAACGTCTAAGTACGATGAAA
GAAGAGTACTTTGAACGCCATCCAGAATCATATGTCACCCATATTGAAGAAATGGCTCCGATTGTTCTTGAATTAACCTC
TTTAGTAAAGGAATTCGGCCGACGATATTCACAAGCAAAACGAGAAAAGTCACTCGTTGATTTCGGAGATCTGGAGCACT
ATTGCCTGCATGTATTAAAAGGTGAAGGATCAACAATGGATCATCCAGTACCATCAGATGCTGCAAATGAGTACCGTGAA
CGTTTTGTTGAAGTTTTAGTTGATGAATACCAAGATACAAACTTTGTACAAGAATCAATCGTACGGCTTGTAGCAAAAGA
TGAAGATCAAGGCAACATGTTCATGGTGGGTGATGTAAAACAGTCGATCTATCGCTTTCGGTTAGCTGAACCACTGCTTT
TTTTATCAAAATATAAGCAGTTTACAAAAGAAGGTGGAGCAGGTCTCAGAATTGATCTCGCTAAAAACTTCAGAAGCCGT
GAAAGTGTGCTTCACGCTACTAATTATCTGTTCAGACAGATTATGAATGAGACAGTTGGAGAGATCGAATATAGTGAAGA
TGCTGAACTTAAGCTAGGCGCATCTTATTATCCTGAGTTCGAAAACAATGAGCCAGAATTAATTTTAATAAATAAAAGCG
AGCCGGCAGTTGATGAAGAAGGTTTGGATACAGGTGAAATGGAAAAGTCTGAGCTTGAAACCGTTCAGCTTGAAGCAAGA
GTAATCGCTGAAAAGATTAAAGAAATTATTGGTGCAAATGGTGAACCTCCACACCTTGTTTTTGATAAATATACAAAATC
GATGCGTCCTGCAAAATATAAAGATATCGTTATGCTTTTTCGTGCAACATCAGCTTGGGCTCCTGTTATTTTGGAAGAGT
TTAAACAACAAGGAATTCCTGCTTATGCTGAATTATCTACTGGATATTTCGATGCAACCGAAATTCATGTTATGATGTCT
CTTTTAAAAGTGATCGATAATCCGCTGCAGGATATTCCCTTGGCTTCCGTTCTCCGTTCACCACTAGTTGGCATGTCTGG
AGAAGAGCTTGCTATTGTACGCCTTATCCAAAAAAAGGATAACTACCTTGGAGCTGCCGAGCAGTACAGTAAGAGCGGAA
GTGATCCTGCTATTCGTGAAAAGGTTTCGTTCTTTTTAGAAAAGCTGGATACATGGAGGAATCAGGCCAGACAAGGCTCA
CTCGCAGACTTGATCTGGGATATTTATAGAGAAACAGGCTATTTTGACTATGTCGGCGGCCTTCCCGGAGGAAAGCAAAG
ACAAGCTAATTTAAGAGCGTTGTACGACCGGGCTAAAATGTATGAGAGTACATCTTTCCGCGGACTTTTCCGCTTTCTTC
GTTTTATTGAAAGAATGCAGGAACGTGGAAAAGATTTAGGGGCCGCTAGAGCACTTGGTGAGCAAGAAGATGTAGTTAGG
ATCATGACGATTCATAAAAGTAAAGGGCTGGAGTTTCCGATTGTGTTCGTAGGAGGCTTGAATAAACAGTTCAATACACG
GGATCTAAAAGAAAAGGTGCTGCTTCATAAAGAGCTTGGTCTCGGAACGCAATATATCAATCCGCAATACCGTGTGATGT
ACCCGACACTTCCGAAGCTTGCAATTACAGGGCGCAAGAAGATGGAACTCATAGCAGAAGAGATGCGAGTTCTCTATGTG
GCGCTGACGCGTGCAAAAGAGAAATTGTATCTAATTGGAACAGTGAATGATATTGAGAAATCAGCAGACAAATGGAGTGA
TTCCCTTTATGAGGAAAGCTGGCTGTTACCTGATTTTGAGCGTACCCAAGTAATGAGTTATCTTGATTGGATTGGACCAG
CCGTTATCCGTCATAAAAATGCAGCGCCGATATTGGCTCATTTACAATCTAGTGAACAGGGCCGTGGGGAAGTGTTCATC
GATCAGGCAGACTGGAAAATTACGATTCTCCCAGCTAGTGATTATGAGGAACTGAATATTGAACAAAAACAAGATCAGCA
AGAGCTGATAACGACGCTGCAAAAAGGTGAAACAGTTGGATTAACGAGTGAAGCTAATGAAAAAATCATACAGCGCCTAG
AGTGGAGATATGAATATTCACAGTCGTCTGTTACCCGATCTAAGCAATCTGTTACCGAGATAAAAAAGCAGTTTCAAAGC
CATGATGAATACAGCGGTACCGACTTGGTAAAAGTGGCACAGCCTGTCATCCACGAACGTCCGAGATTTATGCAGGAAGC
ACAGCTGAATGCAGCAGAAGCAGGGACAGCGATGCACATGGTTATGCAGCACATTGATTTCAACTCATCTATATCATTAG
AAGATTTGGAAGAACAACTTGCGGCAATGACTGCAAAAGAACTCTTAACTGAAGAACAAGCTCGTTACATTCAACTTCCG
CAAATTAAAGCTTTCATAGAAGGTCCATTAGGAAGAAGAATCATCGAGATTGGAAACATTCATCGAGAGATCCCTTTCAG
TCTGGCACTTCCTTTGTCAGAAACAAAGACAGGATGGAGCGGGGAGGAAGATGAGCACATACTCGTTCAAGGTGTGATAG
ATTGTTTAGTTGAAGAAGACGATGGTTTTATATTAGTTGATTATAAAACAGACAAAATTACAAATCGATTTACTAACGGA
TTCATAGGTGCAAAACAAGTGCTGCTTGATCGTTATGAAGTGCAGCTTACTTTATATGCAAAGGCGATTGAACGGATTTT
AAAGAAGCCTGTAAAAGAAAGCTATCTGTACTTTTTTGATGGCGGACATATTTTACAAGTTAAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1066-1237)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-894)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (13-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	51.356	100	0.516