Detailed information NTG0878383

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: RGB74_RS12220
• Genome accession: NZ_CP134049
• Coordinates: 2474509..2478012 (-)
• Length: 1167 a.a.
• NCBI ID: WP_310759579.1
• Uniprot ID: -
• Organism: Bacillus sp. NEB1478
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2469509..2483012

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGB74_RS12210 (RGB74_12210)	-	2469574..2470754 (-)	1181	Protein_2425	exonuclease SbcCD subunit D	-
RGB74_RS12215 (RGB74_12215)	addA	2470766..2474512 (-)	3747	WP_310759578.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RGB74_RS12220 (RGB74_12220)	addB	2474509..2478012 (-)	3504	WP_310759579.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RGB74_RS12225 (RGB74_12225)	-	2478291..2479349 (-)	1059	WP_310759580.1	GGDEF domain-containing protein	-
RGB74_RS12230 (RGB74_12230)	-	2479467..2480402 (-)	936	WP_310759581.1	GGDEF domain-containing protein	-
RGB74_RS12235 (RGB74_12235)	-	2480775..2481428 (-)	654	WP_310759582.1	serine/threonine protein kinase	-
RGB74_RS12240 (RGB74_12240)	-	2481535..2481894 (-)	360	WP_310759583.1	hypothetical protein	-
RGB74_RS12245 (RGB74_12245)	-	2481908..2482117 (-)	210	WP_310759584.1	hypothetical protein	-

Sequence

Protein

• Length: 1167 a.a.
• Molecular weight: 132834.40 Da
• Isoelectric Point: 6.7150

>NTDB_id=878383 RGB74_RS12220 WP_310759579.1 2474509..2478012(-) (addB) [Bacillus sp. NEB1478]
MSLQFILGRSGSGKSHSVFQDIQQKLADDPSGSPIIYLVPDQMTFQSEYKLAGTPGLNGMMRAQVYSFSRLAWRILGETG
GMTRLHITSSGVRMMLRKIIENKKEELKIFKKASEQNGFYELLEMMMTELKRYCVSSEHLTYNAETLLSLGQKENNKKVL
KDKLSDLSVISSALEKALAEQYLDSEDYLRLLQEKIAYSEMIKNCEIYIDGFHSFTPQELEVTAALMQHAKKVTIALTVD
PSQENGSEPHELDVFYMTIRTRQVLSQKAQELGVELMEPVLRYETPKFRSHAISHIEKHYDTRPAMTFPDSSGIHIGAAV
NLRLEVEGAARKIIELVRDHGYRYRDIAVTVRNSGSYQPLIETVFADYDIPVFLDQKKSMLHHPLIELIRSSLDIVLKNW
RYEAVFRAVKTELLYPADEISKTTTEIRRGMDELENYVLALGIQGSRWTDSKPWQYKRFRGFEAEDFGKTTRDDEKEQLF
NDLREMIVSPLKRLSRRLSSSKTIKDYCSALYTFLEELSIPLKLEKMRFRSESDGKLRAAREHGQVWNAVLNLLDEMVEL
TGKEELSKETWTQMLDTGLESMKFALVPPSLDQVLAGTIERSRFTDVKVNFILGVNDGVIPAKPKDDGLLSEDERDALSK
NGVELAPSSRRKLLDEQFMIYLALTNASDRLYVSYALADEEGKTLQPSMVIGRLQDLFPALNEELYQNEPSIEGSMDFIQ
HPLKSISCLSVQLREWKRGYPISPIWWDVYNWSITNHKYGHYAAKTISSLFYLNREAKLSEEVSRELYGETILTSISRME
LFQSCPFSQFASYGLKLKERQMYKLEAPDIGQLFHAALKQMNDALQQRRVSWSDLSKGDCYRMAGDIVELLAPQLQHEIL
LSSNRHLYIKKKLKEVVGKASHVLSEHAKASGFTPAGLELAFGTGGTLPPLMYKLPNGTTMEVVGRIDRVDTAESSNGLL
MRIIDYKSSSTGLSLSELYHGIALQMLTYLDVAVTHSEKWLGKESIPAGVLYFHVHNPLLNKKKLLSVEEIEQEILKKFK
MKGLVLADEEAVNLMDSTMEKRSNIVPVALTAKGFHKTQSSVATSPHFQTMRNHVRKKAVDSGAGITNGIIDISPYEMSK
KTPCTYCSYKPVCQFDQTLDENQYRQLRSEKDEIILEKMMQEGGDLI

Nucleotide

• Length: 3504 bp

>NTDB_id=878383 RGB74_RS12220 WP_310759579.1 2474509..2478012(-) (addB) [Bacillus sp. NEB1478]
ATGAGTTTGCAATTTATTTTGGGGCGATCCGGCAGCGGAAAGTCCCACTCTGTCTTTCAGGACATCCAACAAAAGTTGGC
AGATGATCCATCTGGAAGTCCTATTATTTATTTAGTACCAGATCAGATGACATTTCAATCAGAATACAAACTTGCAGGTA
CACCAGGTTTGAACGGAATGATGAGAGCCCAAGTCTATAGTTTCTCAAGGCTAGCCTGGCGTATTCTTGGTGAAACAGGG
GGTATGACGCGGCTTCACATCACAAGTTCTGGAGTACGGATGATGCTCAGAAAGATTATTGAGAACAAAAAAGAAGAACT
TAAGATCTTTAAGAAAGCATCAGAGCAGAATGGTTTTTATGAATTACTAGAAATGATGATGACAGAGCTGAAGCGATATT
GTGTATCTTCAGAGCATTTAACTTATAATGCAGAAACGCTTTTAAGTCTTGGTCAGAAAGAAAACAATAAGAAGGTACTG
AAAGATAAATTATCCGATTTGAGCGTAATTTCAAGTGCGCTGGAAAAGGCGCTGGCTGAACAATATTTAGATTCAGAAGA
TTACTTGAGATTGCTGCAGGAAAAAATCGCTTATTCAGAAATGATCAAGAACTGTGAAATTTATATAGACGGTTTTCACT
CGTTTACACCTCAGGAGCTAGAAGTAACAGCAGCATTGATGCAGCATGCGAAAAAAGTAACCATTGCACTTACTGTAGAT
CCATCACAAGAAAATGGGTCTGAACCTCATGAATTAGATGTGTTTTATATGACGATAAGAACTAGGCAAGTATTATCACA
AAAAGCACAAGAACTTGGTGTGGAATTGATGGAGCCGGTTTTACGCTATGAAACTCCGAAATTCAGATCGCATGCTATTT
CACATATTGAAAAACATTATGATACAAGACCTGCAATGACTTTTCCAGATTCATCAGGAATACATATTGGAGCAGCGGTT
AATTTAAGATTAGAAGTAGAAGGAGCAGCACGGAAGATTATTGAGCTGGTTCGTGATCATGGATACAGATATCGTGATAT
CGCCGTAACTGTCCGTAACTCAGGAAGCTACCAGCCATTGATCGAAACCGTTTTTGCCGATTATGACATTCCTGTATTTT
TAGATCAGAAAAAATCGATGCTTCATCATCCGCTGATCGAATTGATTCGTTCTTCCTTAGATATTGTGCTGAAAAATTGG
AGGTATGAGGCAGTTTTCCGAGCGGTCAAAACAGAATTGCTATACCCGGCTGATGAGATCAGCAAAACGACAACAGAGAT
CAGAAGAGGTATGGACGAACTAGAAAACTATGTACTTGCTCTTGGTATACAAGGAAGTAGATGGACAGACTCTAAACCAT
GGCAATATAAGAGATTCAGAGGGTTTGAAGCCGAAGATTTTGGTAAAACTACACGCGATGATGAGAAAGAACAACTATTC
AATGATTTGCGTGAAATGATTGTTTCACCATTAAAACGCTTGTCTAGGAGACTGTCTTCAAGTAAGACGATTAAAGATTA
TTGCAGTGCGCTTTATACATTTCTAGAGGAGCTCAGCATCCCTTTAAAACTTGAAAAAATGAGATTCAGATCCGAATCTG
ACGGGAAACTTCGGGCAGCAAGAGAGCACGGTCAAGTGTGGAATGCTGTGCTGAACCTATTAGATGAGATGGTTGAACTA
ACCGGAAAAGAAGAACTTTCAAAAGAAACTTGGACGCAGATGCTGGATACAGGGCTAGAAAGCATGAAATTTGCATTAGT
TCCTCCATCACTGGATCAAGTTCTTGCAGGAACAATCGAACGATCCCGATTTACTGATGTAAAAGTGAATTTTATTTTAG
GTGTTAACGATGGTGTTATTCCTGCAAAACCAAAAGATGACGGTCTATTATCTGAAGATGAACGAGATGCTCTTTCCAAA
AATGGTGTCGAACTTGCACCTTCATCACGCCGTAAATTGCTTGATGAACAGTTTATGATTTATTTGGCTTTAACAAATGC
CAGTGACCGTCTATATGTGTCGTATGCTCTTGCCGATGAAGAAGGGAAAACCCTGCAGCCCTCAATGGTTATTGGGCGTC
TTCAAGACCTTTTTCCGGCATTAAATGAAGAGCTGTATCAAAATGAACCATCTATAGAAGGAAGTATGGACTTTATTCAA
CATCCTTTAAAAAGTATATCCTGCCTAAGCGTTCAGCTTCGCGAATGGAAAAGGGGATATCCGATTTCGCCGATATGGTG
GGATGTGTACAATTGGTCCATAACTAATCATAAATATGGCCATTATGCTGCAAAAACGATATCCAGTTTGTTTTATTTAA
ACCGAGAAGCGAAATTAAGCGAAGAAGTTTCCAGAGAACTGTACGGTGAAACAATCCTAACAAGTATTTCGCGCATGGAA
CTTTTTCAGTCTTGTCCGTTTTCTCAATTTGCATCGTATGGATTGAAATTGAAGGAGCGGCAGATGTACAAGCTCGAAGC
TCCCGATATCGGACAGCTTTTTCATGCTGCGTTAAAGCAGATGAACGATGCATTGCAGCAGAGAAGAGTGTCTTGGAGCG
ATCTCTCAAAAGGGGATTGTTACCGTATGGCTGGAGATATTGTAGAATTATTGGCTCCACAGCTTCAGCATGAAATCTTA
CTGAGTTCGAATCGGCACCTTTATATAAAAAAGAAATTGAAAGAAGTGGTCGGGAAAGCTTCTCATGTGCTGAGTGAGCA
TGCAAAAGCAAGCGGTTTTACGCCGGCCGGACTGGAACTTGCGTTTGGGACAGGCGGGACATTGCCGCCTCTCATGTACA
AACTTCCAAACGGTACAACCATGGAAGTTGTCGGCCGTATTGATAGGGTCGATACAGCAGAAAGTTCTAATGGGTTATTA
ATGCGTATTATCGATTATAAATCCAGCTCTACTGGATTGAGTTTATCTGAGCTTTATCATGGAATTGCGCTGCAAATGCT
TACGTATCTTGATGTAGCAGTTACTCATTCAGAAAAGTGGCTTGGTAAAGAATCGATACCGGCAGGTGTGCTTTATTTTC
ATGTTCATAATCCATTGCTGAATAAGAAGAAGTTATTATCTGTCGAAGAGATTGAGCAGGAAATTTTGAAAAAGTTCAAA
ATGAAGGGTCTTGTGCTAGCAGATGAAGAAGCGGTAAACCTGATGGATAGCACAATGGAGAAACGTTCAAATATCGTTCC
AGTTGCTCTGACGGCAAAAGGATTTCACAAAACACAGTCTTCTGTTGCAACTAGCCCGCATTTTCAAACTATGCGTAACC
ATGTGCGCAAGAAAGCAGTGGACAGCGGTGCAGGAATTACGAATGGAATCATTGATATATCGCCATATGAGATGAGTAAA
AAAACGCCTTGTACGTATTGTTCTTATAAACCTGTCTGTCAGTTTGATCAGACACTCGATGAAAATCAGTATCGTCAGCT
AAGATCAGAAAAAGATGAAATTATTTTAGAGAAAATGATGCAGGAAGGAGGGGACTTAATTTGA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (795-1134)

• UvrD-like helicase C-terminal domain (UvrD_C) (293-676)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	50.47	100	0.506