Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   RGB74_RS10375 Genome accession   NZ_CP134049
Coordinates   2108830..2110071 (-) Length   413 a.a.
NCBI ID   WP_310759243.1    Uniprot ID   -
Organism   Bacillus sp. NEB1478     
Function   unknown (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2061678..2112204 2108830..2110071 within 0


Gene organization within MGE regions


Location: 2061678..2112204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGB74_RS10155 (RGB74_10155) - 2062802..2064553 (-) 1752 WP_310759202.1 ABC transporter transmembrane domain-containing protein -
  RGB74_RS10160 (RGB74_10160) mnmH 2064650..2065699 (-) 1050 WP_310759203.1 tRNA 2-selenouridine(34) synthase MnmH -
  RGB74_RS10165 (RGB74_10165) - 2065793..2066011 (-) 219 WP_066241071.1 YneF family protein -
  RGB74_RS10170 (RGB74_10170) sirA 2066090..2066530 (-) 441 WP_310759204.1 sporulation inhibitor of replication protein SirA -
  RGB74_RS10175 (RGB74_10175) tkt 2066685..2068688 (-) 2004 WP_396136061.1 transketolase -
  RGB74_RS10180 (RGB74_10180) - 2068863..2069147 (-) 285 WP_310759206.1 iron-sulfur cluster biosynthesis family protein -
  RGB74_RS10185 (RGB74_10185) - 2069252..2069491 (-) 240 WP_310759207.1 DUF896 domain-containing protein -
  RGB74_RS10190 (RGB74_10190) - 2069551..2070195 (-) 645 WP_310759208.1 recombinase family protein -
  RGB74_RS10195 (RGB74_10195) - 2070215..2070481 (-) 267 WP_310759209.1 LysM peptidoglycan-binding domain-containing protein -
  RGB74_RS10200 (RGB74_10200) lexA 2070643..2071257 (+) 615 WP_310759210.1 transcriptional repressor LexA -
  RGB74_RS10205 (RGB74_10205) - 2071455..2071841 (-) 387 WP_310759211.1 DUF1259 domain-containing protein -
  RGB74_RS10210 (RGB74_10210) - 2072263..2073303 (+) 1041 WP_310759212.1 glycosyltransferase -
  RGB74_RS10215 (RGB74_10215) - 2073300..2074094 (+) 795 WP_310759213.1 sugar phosphate nucleotidyltransferase -
  RGB74_RS10220 (RGB74_10220) - 2074084..2075250 (+) 1167 WP_310759214.1 glycosyltransferase family 2 protein -
  RGB74_RS10225 (RGB74_10225) - 2075617..2076819 (+) 1203 WP_310759215.1 glycosyltransferase family 2 protein -
  RGB74_RS10230 (RGB74_10230) - 2077086..2077961 (-) 876 WP_310759216.1 LysR family transcriptional regulator -
  RGB74_RS10235 (RGB74_10235) - 2078148..2078312 (+) 165 WP_310759217.1 hypothetical protein -
  RGB74_RS10240 (RGB74_10240) - 2078813..2079331 (-) 519 WP_310759218.1 AAA family ATPase -
  RGB74_RS10245 (RGB74_10245) - 2079576..2080244 (-) 669 WP_310759219.1 Type 1 glutamine amidotransferase-like domain-containing protein -
  RGB74_RS10250 (RGB74_10250) - 2080355..2080813 (-) 459 WP_310759220.1 SRPBCC family protein -
  RGB74_RS10255 (RGB74_10255) - 2080829..2081329 (-) 501 WP_310759221.1 MerR family transcriptional regulator -
  RGB74_RS10260 (RGB74_10260) glnA 2081752..2083086 (-) 1335 WP_310759222.1 type I glutamate--ammonia ligase -
  RGB74_RS10265 (RGB74_10265) - 2083157..2083549 (-) 393 WP_310759223.1 MerR family transcriptional regulator -
  RGB74_RS10270 (RGB74_10270) - 2083672..2084940 (-) 1269 WP_310759224.1 methionine gamma-lyase family protein -
  RGB74_RS10275 (RGB74_10275) hflX 2084942..2086198 (-) 1257 WP_310759225.1 GTPase HflX -
  RGB74_RS10280 (RGB74_10280) spoVK 2086238..2087188 (-) 951 WP_310759226.1 stage V sporulation protein K -
  RGB74_RS10285 (RGB74_10285) - 2087460..2088080 (+) 621 WP_310759227.1 YitT family protein -
  RGB74_RS10290 (RGB74_10290) - 2088207..2088800 (-) 594 WP_310759228.1 YhcN/YlaJ family sporulation lipoprotein -
  RGB74_RS10295 (RGB74_10295) - 2088906..2089883 (+) 978 WP_310759229.1 tyrosine-type recombinase/integrase -
  RGB74_RS10300 (RGB74_10300) hfq 2089936..2090172 (-) 237 WP_251322232.1 RNA chaperone Hfq -
  RGB74_RS10305 (RGB74_10305) miaA 2090204..2091154 (-) 951 WP_310759230.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  RGB74_RS10310 (RGB74_10310) mutL 2091164..2093035 (-) 1872 WP_310759231.1 DNA mismatch repair endonuclease MutL -
  RGB74_RS10315 (RGB74_10315) mutS 2093050..2095644 (-) 2595 WP_310759232.1 DNA mismatch repair protein MutS -
  RGB74_RS10320 (RGB74_10320) - 2096103..2096675 (-) 573 WP_310759233.1 outer spore coat protein CotE -
  RGB74_RS10325 (RGB74_10325) - 2097208..2097654 (-) 447 WP_310759234.1 RicAFT regulatory complex protein RicA family protein -
  RGB74_RS10330 (RGB74_10330) miaB 2097654..2099186 (-) 1533 WP_396135959.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  RGB74_RS10335 (RGB74_10335) - 2099521..2100696 (-) 1176 WP_310759236.1 glycine C-acetyltransferase -
  RGB74_RS10340 (RGB74_10340) tdh 2100714..2101754 (-) 1041 WP_310759237.1 L-threonine 3-dehydrogenase -
  RGB74_RS10345 (RGB74_10345) - 2101936..2102802 (-) 867 WP_310759238.1 2-oxoacid:ferredoxin oxidoreductase subunit beta -
  RGB74_RS10350 (RGB74_10350) - 2102804..2104546 (-) 1743 WP_310759239.1 2-oxoacid:acceptor oxidoreductase subunit alpha -
  RGB74_RS10355 (RGB74_10355) spoVS 2104957..2105217 (-) 261 WP_010193268.1 stage V sporulation protein SpoVS -
  RGB74_RS10360 (RGB74_10360) - 2105205..2105330 (-) 126 WP_310759240.1 hypothetical protein -
  RGB74_RS10365 (RGB74_10365) rny 2105802..2107367 (-) 1566 WP_310759241.1 ribonuclease Y -
  RGB74_RS10370 (RGB74_10370) recA 2107659..2108699 (-) 1041 WP_310759242.1 recombinase RecA Machinery gene
  RGB74_RS10375 (RGB74_10375) cinA 2108830..2110071 (-) 1242 WP_310759243.1 competence/damage-inducible protein A Machinery gene
  RGB74_RS10380 (RGB74_10380) pgsA 2110229..2110807 (-) 579 WP_310759244.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  RGB74_RS10385 (RGB74_10385) - 2110850..2111704 (-) 855 WP_310759245.1 RodZ domain-containing protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45336.99 Da        Isoelectric Point: 5.7999

>NTDB_id=878377 RGB74_RS10375 WP_310759243.1 2108830..2110071(-) (cinA) [Bacillus sp. NEB1478]
MNAEIIAVGSELLLGQIVNSNAQFISKNLADIGVNVFYHSVAGDNAKRLASVIQIAKTRSNLLIFTGGLGPTKDDLTKET
IADVFQKELVHDERSLQYIKNYFVQTNRKMTPNNEKQALIIKDSVVFPNENGMAPGMAFSDEGMTCILLPGPPSEMKPMF
QKFVIPFLESQSETKTKLYSRVLKFFGIGESALETELQDLIEKQSNPTIAPLASEHEVTIRLTAVHSVQLEAEKLLDETE
EKILSKTSSYFYGYDLDTLEGCVIDSLLKKNATIASAESLTGGLFSKVITDIPGASAIFKGGAVVYSNELKQTLLGVPEK
LLKQHGAVSKECAEWMAEKIRNMTNADYGISFTGVAGPEKQEEKNAGTVFIAISNAGETISFSLQLAGSRKAIRERTVKY
GFFYMNQMVKKGQ

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=878377 RGB74_RS10375 WP_310759243.1 2108830..2110071(-) (cinA) [Bacillus sp. NEB1478]
ATGAACGCAGAGATCATAGCTGTAGGAAGTGAATTGCTTTTAGGGCAAATTGTTAATTCGAATGCTCAATTTATCTCGAA
AAACCTAGCAGATATCGGTGTGAATGTATTTTACCATTCTGTAGCAGGAGATAATGCCAAGAGGCTTGCATCTGTCATTC
AAATTGCCAAAACACGATCAAACCTTTTGATTTTCACGGGAGGACTTGGACCTACTAAAGATGACTTGACGAAAGAAACG
ATTGCTGATGTTTTTCAAAAAGAGCTTGTGCATGACGAACGATCACTGCAGTACATAAAAAATTATTTTGTACAGACTAA
TCGAAAGATGACGCCTAATAATGAGAAGCAAGCGTTGATTATTAAAGATTCTGTAGTATTCCCAAATGAAAATGGAATGG
CCCCTGGGATGGCATTTTCTGATGAAGGAATGACTTGTATCTTGTTGCCTGGCCCTCCGAGCGAAATGAAACCGATGTTT
CAAAAATTCGTGATTCCATTTTTGGAGTCTCAATCCGAAACGAAAACAAAACTTTATTCCCGTGTATTAAAGTTTTTTGG
AATCGGGGAATCTGCATTGGAAACAGAGCTTCAAGATTTAATCGAAAAACAAAGTAACCCAACCATCGCACCATTAGCTT
CAGAGCATGAAGTAACAATTCGATTAACGGCTGTTCATTCTGTTCAACTTGAAGCAGAAAAACTGCTGGACGAGACGGAG
GAAAAAATATTAAGCAAAACATCCTCCTATTTTTATGGGTATGATCTTGATACATTAGAGGGTTGTGTGATAGATTCATT
GCTTAAGAAAAATGCCACTATTGCGTCAGCCGAGAGTTTAACAGGCGGACTTTTTAGCAAAGTTATTACGGATATCCCTG
GTGCATCTGCTATTTTTAAGGGAGGCGCAGTTGTTTATTCGAATGAATTAAAACAAACCCTTCTTGGTGTGCCTGAGAAA
CTTTTGAAGCAGCATGGTGCTGTGAGTAAGGAATGCGCTGAATGGATGGCTGAAAAAATTAGAAACATGACAAATGCAGA
CTATGGAATAAGTTTTACAGGAGTAGCAGGTCCTGAGAAACAAGAAGAGAAAAATGCAGGAACTGTATTTATAGCGATTT
CTAATGCCGGAGAAACAATATCTTTCTCTTTACAGCTTGCAGGCTCAAGAAAAGCTATTCGGGAGAGAACGGTAAAATAC
GGATTTTTCTATATGAATCAAATGGTCAAAAAAGGTCAATGA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-169)

Competence-damaged protein (CinA)

(258-407)

Damage-inducible protein CinA KH domain (CinA_KH)

(180-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Bacillus subtilis subsp. subtilis str. 168

53.545

99.031

0.53
  cinA Streptococcus mutans UA159

48.068

100

0.482
  cinA Streptococcus mitis SK321

47.573

99.758

0.475
  cinA Streptococcus mitis NCTC 12261

47.087

99.758

0.47
  cinA Streptococcus pneumoniae TIGR4

46.845

99.758

0.467
  cinA Streptococcus pneumoniae Rx1

46.359

99.758

0.462
  cinA Streptococcus pneumoniae R6

46.359

99.758

0.462
  cinA Streptococcus pneumoniae D39

46.117

99.758

0.46
  cinA Streptococcus suis isolate S10

39.078

99.758

0.39