Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   RIM63_RS08195 Genome accession   NZ_CP133952
Coordinates   1684274..1685452 (-) Length   392 a.a.
NCBI ID   WP_317583774.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain HT321     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1654546..1684253 1684274..1685452 flank 21


Gene organization within MGE regions


Location: 1654546..1685452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIM63_RS10460 - 1654546..1654704 (-) 159 Protein_1536 DNA/RNA helicase -
  RIM63_RS07975 (RIM63_07975) - 1654693..1655427 (-) 735 Protein_1537 gp58-like family protein -
  RIM63_RS07980 (RIM63_07980) - 1655437..1656051 (-) 615 WP_317583734.1 hypothetical protein -
  RIM63_RS07985 (RIM63_07985) - 1656563..1656733 (-) 171 Protein_1539 collagen-like protein -
  RIM63_RS07990 (RIM63_07990) - 1656730..1658784 (-) 2055 WP_317583736.1 phage tail spike protein -
  RIM63_RS07995 (RIM63_07995) - 1658781..1659551 (-) 771 WP_317583738.1 distal tail protein Dit -
  RIM63_RS08000 (RIM63_08000) - 1659564..1663664 (-) 4101 WP_317583740.1 phage tail tape measure protein -
  RIM63_RS08005 (RIM63_08005) gpG 1663890..1664192 (-) 303 WP_212573332.1 phage tail assembly chaperone G -
  RIM63_RS08010 (RIM63_08010) - 1664285..1664860 (-) 576 WP_231147596.1 major tail protein -
  RIM63_RS08015 (RIM63_08015) - 1664873..1665253 (-) 381 WP_212573334.1 hypothetical protein -
  RIM63_RS08020 (RIM63_08020) - 1665246..1665644 (-) 399 WP_231147595.1 hypothetical protein -
  RIM63_RS08025 (RIM63_08025) - 1665646..1666008 (-) 363 WP_212573336.1 phage head-tail adapter protein -
  RIM63_RS08030 (RIM63_08030) - 1666001..1666309 (-) 309 WP_231147594.1 hypothetical protein -
  RIM63_RS08035 (RIM63_08035) - 1666309..1666482 (-) 174 WP_212573338.1 hypothetical protein -
  RIM63_RS08040 (RIM63_08040) - 1666493..1667626 (-) 1134 WP_317583744.1 phage major capsid protein -
  RIM63_RS08045 (RIM63_08045) - 1667643..1668449 (-) 807 WP_212573340.1 head maturation protease, ClpP-related -
  RIM63_RS08050 (RIM63_08050) - 1668430..1669617 (-) 1188 WP_212573341.1 phage portal protein -
  RIM63_RS08055 (RIM63_08055) - 1669770..1669982 (-) 213 WP_228294450.1 hypothetical protein -
  RIM63_RS08060 (RIM63_08060) - 1669985..1671715 (-) 1731 WP_317583751.1 terminase large subunit domain-containing protein -
  RIM63_RS08065 (RIM63_08065) - 1671728..1672045 (-) 318 WP_015984806.1 P27 family phage terminase small subunit -
  RIM63_RS08070 (RIM63_08070) - 1672188..1672493 (-) 306 WP_231147590.1 HNH endonuclease -
  RIM63_RS08075 (RIM63_08075) - 1672486..1672872 (-) 387 WP_212573344.1 hypothetical protein -
  RIM63_RS08080 (RIM63_08080) - 1672853..1673137 (-) 285 WP_317583755.1 hypothetical protein -
  RIM63_RS08085 (RIM63_08085) - 1673290..1673865 (-) 576 WP_212573345.1 site-specific integrase -
  RIM63_RS08090 (RIM63_08090) - 1673968..1674369 (-) 402 WP_212573346.1 transcriptional regulator -
  RIM63_RS08095 (RIM63_08095) - 1674372..1674587 (-) 216 WP_212573347.1 hypothetical protein -
  RIM63_RS08100 (RIM63_08100) - 1674604..1675272 (-) 669 WP_317583756.1 DNA methyltransferase -
  RIM63_RS08105 (RIM63_08105) - 1675293..1675880 (-) 588 WP_317583757.1 class I SAM-dependent methyltransferase -
  RIM63_RS08110 (RIM63_08110) - 1675884..1676111 (-) 228 WP_212573350.1 hypothetical protein -
  RIM63_RS08115 (RIM63_08115) - 1676104..1676304 (-) 201 WP_043983617.1 hypothetical protein -
  RIM63_RS08120 (RIM63_08120) - 1676301..1676600 (-) 300 WP_111678836.1 VVA0879 family protein -
  RIM63_RS08125 (RIM63_08125) - 1676642..1677031 (-) 390 WP_111678017.1 YopX family protein -
  RIM63_RS08130 (RIM63_08130) - 1677095..1677307 (-) 213 WP_212573352.1 hypothetical protein -
  RIM63_RS08135 (RIM63_08135) - 1677316..1677456 (-) 141 WP_142240698.1 hypothetical protein -
  RIM63_RS08140 (RIM63_08140) - 1677453..1677686 (-) 234 WP_212573353.1 hypothetical protein -
  RIM63_RS08145 (RIM63_08145) - 1677667..1678050 (-) 384 WP_154803901.1 DnaD domain-containing protein -
  RIM63_RS08150 (RIM63_08150) - 1678227..1678472 (-) 246 WP_012678836.1 helix-turn-helix domain-containing protein -
  RIM63_RS08155 (RIM63_08155) - 1678676..1678828 (-) 153 WP_317583763.1 hypothetical protein -
  RIM63_RS08160 (RIM63_08160) - 1678833..1678988 (-) 156 WP_317583764.1 BOW99_gp33 family protein -
  RIM63_RS08165 (RIM63_08165) - 1679139..1679909 (-) 771 WP_317583765.1 polymer-forming cytoskeletal protein -
  RIM63_RS08170 (RIM63_08170) - 1680054..1680269 (-) 216 WP_212573355.1 helix-turn-helix transcriptional regulator -
  RIM63_RS08175 (RIM63_08175) - 1680457..1681200 (+) 744 WP_317583768.1 S24 family peptidase -
  RIM63_RS08180 (RIM63_08180) - 1681260..1681730 (+) 471 WP_043052995.1 DUF6978 family protein -
  RIM63_RS08185 (RIM63_08185) - 1681756..1682532 (+) 777 WP_043052994.1 DUF1828 domain-containing protein -
  RIM63_RS08190 (RIM63_08190) - 1682820..1684253 (+) 1434 WP_317583772.1 recombinase family protein -
  RIM63_RS08195 (RIM63_08195) comFA/cflA 1684274..1685452 (-) 1179 WP_317583774.1 DEAD/DEAH box helicase Machinery gene

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 44440.85 Da        Isoelectric Point: 9.5707

>NTDB_id=877777 RIM63_RS08195 WP_317583774.1 1684274..1685452(-) (comFA/cflA) [Streptococcus equi subsp. zooepidemicus strain HT321]
MENIENYYGRLLPEGQCPKAVSVWACSLQSMIAKKGTLYCQRCSSLIEKAHQLPSGAYYCRACLVFGRNQSDRPLLYFPP
APFPKGHYLRWQGQLTTYQVAVSHQLTNHVKLKQDTLVHAVTGAGKTEMMYEAIAAVVDKGGWVCIASPRVDVCIELEKR
LSRDFSCQICLMHAESEAYHRSPIIIATTHQLMTFYHAFDLLIIDEVDAFPFVNNRQLNHAAHQAAKADAVTVYLTATST
RDLERKVKQKELVKLTLARRFHGKPLVVPKYQRLFSLLEAINRGKLPRRLITLVKKQRATGYPLLIFFPLIELAEQCCQL
LQKYFPKETIAHASSQSSNRMAIIEQFRQGQITILISTTILERGVTFPTVDVFVLLANHRLYTSSSLIQIKG

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=877777 RIM63_RS08195 WP_317583774.1 1684274..1685452(-) (comFA/cflA) [Streptococcus equi subsp. zooepidemicus strain HT321]
ATGGAGAATATCGAAAATTACTATGGACGTCTTTTACCCGAAGGGCAATGCCCAAAGGCTGTTTCTGTCTGGGCTTGTAG
CTTACAAAGCATGATCGCTAAAAAAGGAACATTATACTGCCAACGCTGTAGCAGTTTAATTGAGAAAGCTCATCAGCTAC
CTAGCGGTGCTTACTACTGTAGAGCCTGTCTCGTTTTTGGTCGAAACCAATCTGATCGGCCCTTGCTCTATTTTCCACCG
GCTCCTTTTCCAAAGGGACATTATCTGAGGTGGCAAGGACAGCTCACAACATATCAGGTAGCTGTCTCTCATCAGCTTAC
TAACCATGTTAAGCTCAAGCAAGACACACTGGTTCATGCCGTTACCGGCGCTGGTAAAACAGAGATGATGTATGAAGCTA
TTGCAGCAGTTGTTGATAAGGGTGGCTGGGTCTGCATTGCTAGTCCACGAGTTGATGTCTGCATAGAGCTTGAAAAACGA
CTATCGCGGGACTTTTCCTGTCAAATCTGTCTTATGCATGCTGAGTCAGAGGCTTATCATAGAAGTCCCATAATCATTGC
CACAACACATCAATTGATGACCTTTTACCATGCTTTTGATCTGCTCATTATTGACGAAGTAGATGCCTTTCCTTTTGTCA
ACAATCGTCAATTAAACCATGCTGCTCATCAGGCTGCAAAAGCAGATGCAGTGACAGTATACCTAACAGCAACCTCTACA
AGAGACTTAGAGCGCAAGGTCAAGCAAAAAGAGCTTGTCAAATTGACATTAGCAAGAAGATTTCATGGCAAGCCCTTAGT
TGTCCCAAAGTATCAAAGATTATTCTCCCTTTTAGAGGCTATCAATCGCGGGAAATTGCCTAGAAGGTTAATCACCCTAG
TCAAAAAACAAAGGGCAACTGGCTATCCTCTCTTAATCTTTTTTCCGCTTATTGAGCTAGCTGAGCAATGCTGTCAGCTG
CTCCAAAAGTATTTTCCTAAGGAAACTATTGCCCATGCTTCCAGTCAGTCATCAAATCGAATGGCTATCATTGAGCAATT
CAGACAAGGACAAATCACTATACTTATATCAACAACCATTTTGGAAAGAGGTGTGACCTTTCCGACCGTAGATGTCTTTG
TCTTGTTAGCCAATCATCGCCTTTACACAAGCAGCAGTCTTATTCAAATCAAGGGTTGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(111-244)

Helicase conserved C-terminal domain (Helicase_C)

(287-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

52.972

98.724

0.523
  comFA/cflA Streptococcus pneumoniae D39

52.972

98.724

0.523
  comFA/cflA Streptococcus pneumoniae R6

52.972

98.724

0.523
  comFA/cflA Streptococcus pneumoniae TIGR4

52.972

98.724

0.523
  comFA/cflA Streptococcus pneumoniae Rx1

52.972

98.724

0.523
  comFA/cflA Streptococcus mitis NCTC 12261

52.713

98.724

0.52
  comFA Lactococcus lactis subsp. cremoris KW2

46.61

90.306

0.421
  comFA Latilactobacillus sakei subsp. sakei 23K

39.071

93.367

0.365