Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RHS39_RS14590 Genome accession   NZ_CP133900
Coordinates   3130118..3131134 (-) Length   338 a.a.
NCBI ID   WP_017449463.1    Uniprot ID   A0A7Y0SFN2
Organism   Vibrio parahaemolyticus strain TW01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3125118..3136134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS39_RS14565 (RHS39_14565) aroK 3125978..3126496 (-) 519 WP_005381319.1 shikimate kinase AroK -
  RHS39_RS14570 (RHS39_14570) pilQ 3126685..3128433 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  RHS39_RS14575 (RHS39_14575) pilP 3128465..3128980 (-) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  RHS39_RS14580 (RHS39_14580) pilO 3128970..3129563 (-) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  RHS39_RS14585 (RHS39_14585) pilN 3129556..3130134 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  RHS39_RS14590 (RHS39_14590) pilM 3130118..3131134 (-) 1017 WP_017449463.1 type IV pilus biogenesis protein PilM Machinery gene
  RHS39_RS14595 (RHS39_14595) - 3131277..3133847 (+) 2571 WP_005480476.1 penicillin-binding protein 1A -
  RHS39_RS14600 (RHS39_14600) oxyR 3133903..3134811 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  RHS39_RS14605 (RHS39_14605) - 3134982..3135710 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=877446 RHS39_RS14590 WP_017449463.1 3130118..3131134(-) (pilM) [Vibrio parahaemolyticus strain TW01]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=877446 RHS39_RS14590 WP_017449463.1 3130118..3131134(-) (pilM) [Vibrio parahaemolyticus strain TW01]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCACAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCGTTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0SFN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482