Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RHS39_RS14580 Genome accession   NZ_CP133900
Coordinates   3128970..3129563 (-) Length   197 a.a.
NCBI ID   WP_005496571.1    Uniprot ID   A0A0F2HLP7
Organism   Vibrio parahaemolyticus strain TW01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123970..3134563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS39_RS14560 (RHS39_14560) aroB 3124846..3125946 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  RHS39_RS14565 (RHS39_14565) aroK 3125978..3126496 (-) 519 WP_005381319.1 shikimate kinase AroK -
  RHS39_RS14570 (RHS39_14570) pilQ 3126685..3128433 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  RHS39_RS14575 (RHS39_14575) pilP 3128465..3128980 (-) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  RHS39_RS14580 (RHS39_14580) pilO 3128970..3129563 (-) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  RHS39_RS14585 (RHS39_14585) pilN 3129556..3130134 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  RHS39_RS14590 (RHS39_14590) pilM 3130118..3131134 (-) 1017 WP_017449463.1 type IV pilus biogenesis protein PilM Machinery gene
  RHS39_RS14595 (RHS39_14595) - 3131277..3133847 (+) 2571 WP_005480476.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23019.57 Da        Isoelectric Point: 4.9419

>NTDB_id=877444 RHS39_RS14580 WP_005496571.1 3128970..3129563(-) (pilO) [Vibrio parahaemolyticus strain TW01]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDGK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=877444 RHS39_RS14580 WP_005496571.1 3128970..3129563(-) (pilO) [Vibrio parahaemolyticus strain TW01]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTAGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTTACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGGGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2HLP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711