Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RHO09_RS06200 Genome accession   NZ_CP133810
Coordinates   1374655..1375200 (-) Length   181 a.a.
NCBI ID   WP_005755689.1    Uniprot ID   -
Organism   Pasteurella multocida strain W22-3735.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1369655..1380200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHO09_RS06175 (RHO09_06175) - 1369869..1370306 (-) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -
  RHO09_RS06180 (RHO09_06180) - 1370515..1371126 (-) 612 WP_005725137.1 YfgM family protein -
  RHO09_RS06185 (RHO09_06185) hisS 1371145..1372416 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  RHO09_RS06190 (RHO09_06190) ispG 1372446..1373549 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RHO09_RS06195 (RHO09_06195) - 1373576..1374532 (-) 957 WP_310850513.1 helix-turn-helix domain-containing protein -
  RHO09_RS06200 (RHO09_06200) pilF/pilF2 1374655..1375200 (-) 546 WP_005755689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RHO09_RS06205 (RHO09_06205) - 1375239..1376423 (-) 1185 WP_310850516.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RHO09_RS06210 (RHO09_06210) - 1376604..1377857 (-) 1254 WP_005755688.1 AI-2E family transporter -
  RHO09_RS06215 (RHO09_06215) glnB 1377858..1378196 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RHO09_RS06220 (RHO09_06220) mog 1378241..1378819 (-) 579 WP_005725110.1 molybdopterin adenylyltransferase -
  RHO09_RS06225 (RHO09_06225) - 1378845..1379774 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20875.37 Da        Isoelectric Point: 7.8258

>NTDB_id=876999 RHO09_RS06200 WP_005755689.1 1374655..1375200(-) (pilF/pilF2) [Pasteurella multocida strain W22-3735.1]
MNRTFRFGIASIFSLFLCACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=876999 RHO09_RS06200 WP_005755689.1 1374655..1375200(-) (pilF/pilF2) [Pasteurella multocida strain W22-3735.1]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTGTGCTTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464