Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   RHM64_RS20495 Genome accession   NZ_CP133776
Coordinates   4543175..4544767 (+) Length   530 a.a.
NCBI ID   WP_322166109.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4538175..4549767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS20475 (RHM64_20475) pgeF 4539965..4540690 (-) 726 WP_322166103.1 peptidoglycan editing factor PgeF -
  RHM64_RS20480 (RHM64_20480) rluD 4540699..4541661 (-) 963 WP_322166104.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  RHM64_RS20485 (RHM64_20485) - 4541813..4542829 (+) 1017 WP_322166105.1 outer membrane protein assembly factor BamD -
  RHM64_RS20490 (RHM64_20490) - 4542946..4543185 (+) 240 WP_322166108.1 PP0621 family protein -
  RHM64_RS20495 (RHM64_20495) pilS 4543175..4544767 (+) 1593 WP_322166109.1 sensor histidine kinase Regulator
  RHM64_RS20500 (RHM64_20500) pilR 4544770..4546113 (+) 1344 WP_322166110.1 sigma-54 dependent transcriptional regulator Regulator
  RHM64_RS20505 (RHM64_20505) - 4546256..4546840 (+) 585 WP_416194726.1 transcriptional regulator -
  RHM64_RS20510 (RHM64_20510) - 4546833..4547262 (+) 430 Protein_4051 hypothetical protein -
  RHM64_RS20515 (RHM64_20515) thiO 4547325..4548431 (-) 1107 WP_322166111.1 glycine oxidase ThiO -
  RHM64_RS20520 (RHM64_20520) - 4548690..4549244 (+) 555 WP_322166112.1 GspH/FimT family pseudopilin -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58624.88 Da        Isoelectric Point: 6.4503

>NTDB_id=876628 RHM64_RS20495 WP_322166109.1 4543175..4544767(+) (pilS) [Pseudomonas sp. AH2]
MPAEVLPLNGTQGQRILRLYHLYRLAIGITLILLISSNLDNDLLELVNVDLFRSGCWVYLIVNILAGVFLNRPEHAAQIF
ALAMADIVMLSALFYAGGGAPSGMGNLLIASVAISNVLLRGRIGLLIAAAAAIGIIYLTFYLSFNRPSAFNHYFQASMLG
ALSFAAALLVQMLTRRLDLSETLANARAADVDNLEELNALILQRMRTGILVLDARRLVLLANQGALTLMGNEQLIGKTID
AYSPQLVDRLRQWLINPMLRPHSVETSVAGPLLQPSFIALNHGEHRQTLVFLEDLSLISQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLKESEELTTPDRRLSQIIHDQTRRMDQVIENVLQLSRRRQADPQLLDLKSWLENFVDEVRASALAN
QLLHVDIGTGTLSTRMDPEQLTQVLVNLLQNGLRYSAQNHDLAQVWLHLFHDPLSDLPVLEILDNGPGVADEQLPKIFEP
FFTTETKGTGLGLYISRELCESNQAHLDYRPREGGGSCFRITFAHPLKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=876628 RHM64_RS20495 WP_322166109.1 4543175..4544767(+) (pilS) [Pseudomonas sp. AH2]
GTGCCCGCTGAAGTACTTCCACTAAACGGCACCCAAGGCCAGCGCATCCTAAGGCTTTATCATCTTTATCGCCTGGCCAT
CGGCATCACGTTAATCCTGTTGATCTCCAGCAACCTCGACAACGACCTGCTGGAGTTGGTCAACGTAGACCTCTTCAGAT
CGGGTTGCTGGGTTTACCTGATCGTCAACATTTTGGCCGGCGTCTTCCTGAACAGGCCGGAACACGCAGCACAGATTTTT
GCCCTGGCGATGGCCGATATCGTGATGCTGTCCGCGCTGTTCTATGCCGGCGGCGGTGCTCCCAGCGGGATGGGCAACTT
GCTGATCGCCTCGGTGGCCATCAGCAACGTGCTGTTGCGCGGGCGCATAGGCTTGCTGATTGCCGCCGCTGCCGCCATCG
GCATTATCTACCTGACCTTCTACCTCAGCTTCAACCGACCTTCAGCCTTCAATCACTACTTTCAGGCAAGCATGCTCGGT
GCGTTGAGTTTTGCGGCAGCGTTATTGGTGCAAATGCTTACTCGACGTCTGGACCTAAGCGAAACCTTGGCCAACGCGCG
TGCAGCGGATGTCGACAACCTGGAAGAGCTCAATGCGCTGATCTTGCAACGTATGCGTACCGGCATTCTGGTGCTCGACG
CCCGGCGCCTAGTGCTCCTGGCCAACCAAGGGGCATTGACGCTGATGGGTAATGAGCAGTTGATCGGCAAAACCATTGAC
GCTTACTCGCCCCAATTGGTGGATCGTTTACGGCAATGGTTGATCAACCCCATGTTACGTCCGCACAGCGTCGAGACGTC
CGTTGCGGGCCCGCTGCTGCAACCGAGTTTTATCGCCTTAAACCATGGCGAGCACCGACAAACATTGGTGTTCCTCGAAG
ACTTATCGTTGATCTCCCAGCAGGCCCAACAACTTAAACTGGCCGCACTGGGGCGCCTGACGGCCGGTATTGCCCACGAA
ATTCGTAACCCGCTGGGCGCAATTAGTCACGCGGCCCAGTTGCTAAAAGAATCAGAGGAATTGACTACGCCAGACCGTCG
ACTGAGCCAGATCATCCATGACCAAACGCGGCGCATGGATCAAGTCATCGAAAACGTCCTGCAGCTGTCTCGTCGACGTC
AAGCCGACCCTCAGCTGCTTGATCTCAAGAGCTGGCTGGAAAACTTTGTCGACGAGGTCCGCGCCTCAGCGCTTGCCAAT
CAACTGCTGCACGTCGACATCGGAACCGGCACGCTGAGTACACGGATGGACCCCGAACAATTGACCCAAGTGTTGGTCAA
TCTGCTGCAGAACGGCTTGCGCTACAGTGCTCAGAACCACGATCTGGCGCAGGTCTGGCTTCATTTGTTTCACGATCCGC
TCAGCGACTTACCGGTACTTGAAATACTCGATAACGGCCCTGGCGTGGCCGACGAGCAATTACCGAAGATTTTCGAACCG
TTCTTCACCACGGAAACCAAAGGCACCGGCCTGGGCCTTTATATTTCCCGTGAGTTGTGCGAAAGCAATCAGGCGCATCT
GGATTACAGACCTCGGGAAGGCGGCGGCAGCTGTTTTCGCATAACCTTTGCCCATCCGCTGAAGTTGAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

63.585

100

0.636