Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RG463_RS27440 Genome accession   NZ_CP133738
Coordinates   5735074..5735946 (+) Length   290 a.a.
NCBI ID   WP_003094651.1    Uniprot ID   A0AAQ3LID6
Organism   Pseudomonas aeruginosa strain FI-17645     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5730074..5740946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG463_RS27415 (RG463_27415) nadC 5730400..5731248 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG463_RS27425 (RG463_27425) - 5731452..5731916 (-) 465 WP_031630343.1 pilin -
  RG463_RS27430 (RG463_27430) pilB 5732146..5733846 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG463_RS27435 (RG463_27435) pilC 5733850..5735070 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  RG463_RS27440 (RG463_27440) pilD 5735074..5735946 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RG463_RS27445 (RG463_27445) coaE 5735943..5736554 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  RG463_RS27450 (RG463_27450) yacG 5736551..5736751 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RG463_RS27455 (RG463_27455) - 5736788..5736997 (-) 210 WP_003094660.1 hypothetical protein -
  RG463_RS27460 (RG463_27460) - 5737103..5737792 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  RG463_RS27465 (RG463_27465) - 5737789..5738259 (-) 471 WP_003094664.1 hypothetical protein -
  RG463_RS27470 (RG463_27470) - 5738256..5738681 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  RG463_RS27475 (RG463_27475) - 5738814..5739443 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  RG463_RS27480 (RG463_27480) - 5739440..5739889 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  RG463_RS27485 (RG463_27485) - 5739915..5740085 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31852.89 Da        Isoelectric Point: 7.7068

>NTDB_id=876175 RG463_RS27440 WP_003094651.1 5735074..5735946(+) (pilD) [Pseudomonas aeruginosa strain FI-17645]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQANYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=876175 RG463_RS27440 WP_003094651.1 5735074..5735946(+) (pilD) [Pseudomonas aeruginosa strain FI-17645]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGAACTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.516

96.897

0.538
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469