NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	RFN66_RS14360	Genome accession	NZ_CP133705
Coordinates	2793568..2794632 (-)	Length	354 a.a.
NCBI ID	WP_256164105.1	Uniprot ID	-
Organism	Bacillus paralicheniformis strain CP47
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2788568..2799632

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFN66_RS14315 (RFN66_14315)	tapA	2789062..2789790 (-)	729	WP_020452169.1	amyloid fiber anchoring/assembly protein TapA	-
RFN66_RS14320 (RFN66_14320)	-	2790068..2790388 (+)	321	WP_023855188.1	YqzG/YhdC family protein	-
RFN66_RS14325 (RFN66_14325)	-	2790418..2790600 (-)	183	WP_020452171.1	YqzE family protein	-
RFN66_RS14330 (RFN66_14330)	comGG	2790689..2791054 (-)	366	WP_023855189.1	competence type IV pilus minor pilin ComGG	-
RFN66_RS14335 (RFN66_14335)	comGF	2791066..2791554 (-)	489	WP_309539400.1	competence type IV pilus minor pilin ComGF	-
RFN66_RS14340 (RFN66_14340)	comGE	2791463..2791810 (-)	348	WP_023855191.1	competence type IV pilus minor pilin ComGE	-
RFN66_RS14345 (RFN66_14345)	comGD	2791794..2792231 (-)	438	WP_020452175.1	competence type IV pilus minor pilin ComGD	-
RFN66_RS14350 (RFN66_14350)	comGC	2792237..2792530 (-)	294	WP_020452176.1	competence type IV pilus major pilin ComGC	Machinery gene
RFN66_RS14355 (RFN66_14355)	comGB	2792544..2793578 (-)	1035	WP_149169671.1	competence type IV pilus assembly protein ComGB	Machinery gene
RFN66_RS14360 (RFN66_14360)	comGA	2793568..2794632 (-)	1065	WP_256164105.1	competence type IV pilus ATPase ComGA	Machinery gene
RFN66_RS14365 (RFN66_14365)	-	2794790..2795629 (-)	840	WP_039072849.1	STAS domain-containing protein	-
RFN66_RS14375 (RFN66_14375)	-	2795871..2796248 (-)	378	WP_003183482.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
RFN66_RS14380 (RFN66_14380)	-	2796459..2796704 (+)	246	WP_020452180.1	DUF2626 domain-containing protein	-
RFN66_RS14385 (RFN66_14385)	-	2796740..2797378 (-)	639	WP_025811156.1	MBL fold metallo-hydrolase	-
RFN66_RS14390 (RFN66_14390)	-	2797534..2797707 (+)	174	WP_003183486.1	DUF2759 domain-containing protein	-
RFN66_RS14395 (RFN66_14395)	-	2797768..2798082 (-)	315	WP_020452182.1	MTH1187 family thiamine-binding protein	-
RFN66_RS14400 (RFN66_14400)	-	2798098..2799207 (-)	1110	WP_039072850.1	hypothetical protein	-

Sequence

Protein

Download Length: 354 a.a. Molecular weight: 39811.31 Da Isoelectric Point: 9.1063

                                >NTDB_id=875918 RFN66_RS14360 WP_256164105.1 2793568..2794632(-) (comGA) [Bacillus paralicheniformis strain CP47]

MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKERLTRNECSRLISHFKFLSSMDIGERRLPQSGALT

LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY

AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH

AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC

GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide

Download Length: 1065 bp

                                >NTDB_id=875918 RFN66_RS14360 WP_256164105.1 2793568..2794632(-) (comGA) [Bacillus paralicheniformis strain CP47]

TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC

CGGTGAAAAAGAGGCGGTGGTTCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGAAAGATTAACGAGAAATGAGTGCT

CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTGACT

CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT

TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA

AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATCGAATAC

GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA

AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTCG

GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT

GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC

AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC

CTGTGAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC

GGTGACATAAAAACAGAAACATTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA

ACGCTGGATCGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	67.797	100	0.678
pilB	Vibrio cholerae strain A1552	38.671	93.503	0.362

Detailed information NTG0875918