Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   RFN66_RS14355 Genome accession   NZ_CP133705
Coordinates   2792544..2793578 (-) Length   344 a.a.
NCBI ID   WP_149169671.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CP47     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2787544..2798578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN66_RS14305 (RFN66_14305) tasA 2787614..2788408 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  RFN66_RS14310 (RFN66_14310) sipW 2788481..2789065 (-) 585 WP_065644169.1 signal peptidase I SipW -
  RFN66_RS14315 (RFN66_14315) tapA 2789062..2789790 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  RFN66_RS14320 (RFN66_14320) - 2790068..2790388 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  RFN66_RS14325 (RFN66_14325) - 2790418..2790600 (-) 183 WP_020452171.1 YqzE family protein -
  RFN66_RS14330 (RFN66_14330) comGG 2790689..2791054 (-) 366 WP_023855189.1 competence type IV pilus minor pilin ComGG -
  RFN66_RS14335 (RFN66_14335) comGF 2791066..2791554 (-) 489 WP_309539400.1 competence type IV pilus minor pilin ComGF -
  RFN66_RS14340 (RFN66_14340) comGE 2791463..2791810 (-) 348 WP_023855191.1 competence type IV pilus minor pilin ComGE -
  RFN66_RS14345 (RFN66_14345) comGD 2791794..2792231 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  RFN66_RS14350 (RFN66_14350) comGC 2792237..2792530 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  RFN66_RS14355 (RFN66_14355) comGB 2792544..2793578 (-) 1035 WP_149169671.1 competence type IV pilus assembly protein ComGB Machinery gene
  RFN66_RS14360 (RFN66_14360) comGA 2793568..2794632 (-) 1065 WP_256164105.1 competence type IV pilus ATPase ComGA Machinery gene
  RFN66_RS14365 (RFN66_14365) - 2794790..2795629 (-) 840 WP_039072849.1 STAS domain-containing protein -
  RFN66_RS14375 (RFN66_14375) - 2795871..2796248 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RFN66_RS14380 (RFN66_14380) - 2796459..2796704 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  RFN66_RS14385 (RFN66_14385) - 2796740..2797378 (-) 639 WP_025811156.1 MBL fold metallo-hydrolase -
  RFN66_RS14390 (RFN66_14390) - 2797534..2797707 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  RFN66_RS14395 (RFN66_14395) - 2797768..2798082 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39804.10 Da        Isoelectric Point: 9.7341

>NTDB_id=875917 RFN66_RS14355 WP_149169671.1 2792544..2793578(-) (comGB) [Bacillus paralicheniformis strain CP47]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=875917 RFN66_RS14355 WP_149169671.1 2792544..2793578(-) (comGB) [Bacillus paralicheniformis strain CP47]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGTCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGC
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538