Detailed information NTG0875824

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: RFN65_RS08050
• Genome accession: NZ_CP133703
• Coordinates: 1570848..1573178 (+)
• Length: 776 a.a.
• NCBI ID: WP_309530225.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain CP35
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1565848..1578178

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFN65_RS08010 (RFN65_08010)	yhbY	1566085..1566375 (+)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
RFN65_RS08015 (RFN65_08015)	nadD	1566387..1566956 (+)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
RFN65_RS08020 (RFN65_08020)	yqeK	1566946..1567506 (+)	561	WP_015483448.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RFN65_RS08025 (RFN65_08025)	rsfS	1567524..1567880 (+)	357	WP_041333759.1	ribosome silencing factor	-
RFN65_RS08030 (RFN65_08030)	yqeM	1567877..1568620 (+)	744	WP_015384134.1	class I SAM-dependent methyltransferase	-
RFN65_RS08035 (RFN65_08035)	comER	1568686..1569507 (-)	822	WP_063335455.1	late competence protein ComER	-
RFN65_RS08040 (RFN65_08040)	comEA	1569591..1570208 (+)	618	WP_063335454.1	competence protein ComEA	Machinery gene
RFN65_RS08045 (RFN65_08045)	comEB	1570275..1570844 (+)	570	WP_003229978.1	ComE operon protein 2	-
RFN65_RS08050 (RFN65_08050)	comEC	1570848..1573178 (+)	2331	WP_309530225.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RFN65_RS08055 (RFN65_08055)	yqzM	1573218..1573352 (-)	135	WP_003229983.1	YqzM family protein	-
RFN65_RS08060 (RFN65_08060)	-	1573393..1573542 (+)	150	WP_003229985.1	hypothetical protein	-
RFN65_RS08065 (RFN65_08065)	holA	1573582..1574625 (+)	1044	WP_014664666.1	DNA polymerase III subunit delta	-
RFN65_RS08070 (RFN65_08070)	rpsT	1574640..1574906 (-)	267	WP_003229989.1	30S ribosomal protein S20	-
RFN65_RS08075 (RFN65_08075)	gpr	1575110..1576216 (+)	1107	WP_014480309.1	GPR endopeptidase	-
RFN65_RS08080 (RFN65_08080)	spoIIP	1576279..1577484 (+)	1206	WP_014477383.1	spore autolysin SpoIIP	-
RFN65_RS08085 (RFN65_08085)	yqxA	1577501..1577839 (+)	339	WP_014477382.1	YqxA family protein	-

Sequence

Protein

• Length: 776 a.a.
• Molecular weight: 86627.08 Da
• Isoelectric Point: 7.0494

>NTDB_id=875824 RFN65_RS08050 WP_309530225.1 1570848..1573178(+) (comEC) [Bacillus subtilis strain CP35]
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDNIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSAGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVHSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWTNRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVLFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAETLLKHHKIKRLVIPKGFVSEPKDEKVLQTAREEGVTIEEVKRGDVLQIKDLQFHILSPG
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMDVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYNHPHHEVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Nucleotide

• Length: 2331 bp

>NTDB_id=875824 RFN65_RS08050 WP_309530225.1 1570848..1573178(+) (comEC) [Bacillus subtilis strain CP35]
ATGCGTAATTCGCGTTTGTTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCCCGC
TATTTTTCTTTTCATCCTCTTTCTCCTCATCATTTTAATCAAAACGAGGCACGCTTTTCTTATTATTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTGTACTGTATGCAGTCACAGATTCTCAGAATGTCTCTTCCTATCGGCAGGGAACTTATCAATTC
AAGGCAGTGATTGACAATATTCCTAAAATTGACGGCGACCGTATGTCTATGATGGTTGAGACACCTGATAAGGAAAAATG
GGCTGCTGCGTATCGCATTCAGTCTGCTGGTGAAAAAGAACAGCTGTTATACATAGAACCAGGAATGTCATGTGAGTTGA
CTGGTACATTGGAAGAACCGAATCACGCAACTGTGCCGGGTGCATTTGATTATAACGAGTATCTTTATCGGCAGCATATT
CATTGGAATTACTCTGTCACGTCTATCCAAAACTGCAGCGAACCTGAAAATTTCAAGTACAAGGTGCTCAGCTTGAGAAA
ACATATCATATCATTCACAAACAGCCTTCTGCCTCCTGATTCGGCAGGGATTGTACAGGCACTTACAGTTGGTGACAGAT
TTTATGTGGAAGATGAAGTGCTTACTGCGTATCAAAAGCTTGGTGTTGTCCATCTCTTGGCAATATCAGGGCTCCACGTG
GGGATTTTGACAGCAGGTTTGTTTTATATCATGATACGCCTTGGTATAACAAGAGAAAAGGCGTCAATTCTGTTGCTGTT
ATTTCTGCCGCTCTATGTGATGTTGACCGGCGCTGCTCCTTCAGTGCTACGCGCCGCTCTCATGTCGGGTGTTTACTTAG
CTGGAAGCCTTGTCAAATGGCGTGTCCACTCTGCAACTGCAATTTGCCTTTCATACATCGTCCTTCTGCTCTTCAATCCT
TATCATCTCTTTGAAGCCGGTTTTCAGCTATCGTTCGCCGTCAGTTTTTCTTTAATTCTATCCTCTTCTATTTTTCAGCA
GGTTAAAACCTCCTTGGGGCAGCTGACAATTGTATCACTCATCGCTCAGCTGGGCTCGCTTCCGATTCTCCTATATCATT
TTCATCAGTTTTCTATCATCAGCGTACCGATGAATATGTTGATGGTACCATTTTATACCTTCTGTATTTTGCCGGGAGCT
GTAGCAGGTGTTCTTCTATTAAGTCTTTCCGCTTCGTTTGGGAGATTGTTTTTCAGCTGGTTTGATTTATTGATAAGCTG
GACCAATAGGCTAATCACAAACATTGCAGATGTTGATGTATTCACGATTATGATCGCACATCCTGCACCTGTTTTGCTTT
TTTTATTCACGGTCACGATCATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTGTCGCAGTTGATGGTAACCGGAGGC
ATTTGCTGCACGGTGCTGTTTCTGCTCTTTATATATCCGTGTCTTAGTTCCGAAGGAGAAGTGGATATGATAGATATTGG
ACAGGGTGACAGCATGTTTGTAGGTGCTCCGCATCAGCGGGGTCGTGTCTTAATTGATACCGGCGGCACTTTGTCTTACT
CGTCAGAGCCTTGGCGCGAAAAACAGCATCCGTTTTCACTGGGGGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATCAAACAGCTTGACGCTTTAATTCTGACGCACGCTGACCAAGATCATATCGGAGAGGCGGAGACTCTGCTGAAGCATCA
TAAAATAAAGCGCCTCGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGATGAGAAAGTGCTGCAGACAGCCAGAGAAG
AGGGAGTGACAATTGAAGAGGTGAAGCGAGGCGATGTATTGCAAATAAAGGATTTGCAGTTCCATATACTGTCACCTGGA
GCACCTGATCCGGCAAGCAAAAATAATTCCTCTCTCGTTCTGTGGATGGAGACGGGCGGTATGAGCTGGATCTTGACGGG
TGATCTGGAGAAAGAAGGGGAACAGGAGGTGATGGACGTGTTTCCAAATATTAAAGCAGATGTCTTAAAGGTGGGGCACC
ATGGGAGCAAAGGCTCTACCGGTGAAGAATTCATCCAACAGCTTCAGCCGAAAACGGCCATTATCTCAGCCGGGAAAAAC
AATCGGTACAACCATCCTCATCATGAAGTTCTGCAACTATTACAGAGACATTCTATCCGCGTGCTGCGAACAGATCAAAA
CGGAACGATCCAATATAGATACAAAAACAGAGTTGGAACCTTTTCTGTCTATCCTCCATATGATACATCAGATATAACAG
AGACGAACTAA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (507-718)

• Domain of unknown function (DUF4131) (DUF4131) (20-170)

• Competence protein (Competence) (208-469)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	98.067	100	0.981