Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RFN60_RS01980 Genome accession   NZ_CP133653
Coordinates   370681..371130 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain CP38     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365681..376130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS01945 (RFN60_01945) yckA 365832..366512 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  RFN60_RS01950 (RFN60_01950) yckB 366522..367385 (-) 864 WP_029317191.1 transporter substrate-binding domain-containing protein -
  RFN60_RS01955 (RFN60_01955) - 367546..367743 (+) 198 WP_128992271.1 hypothetical protein -
  RFN60_RS01960 (RFN60_01960) yckC 367763..368218 (+) 456 WP_048654748.1 RDD family protein -
  RFN60_RS01965 (RFN60_01965) yckD 368300..368632 (+) 333 WP_015252849.1 YckD family protein -
  RFN60_RS01970 (RFN60_01970) bglC 368786..370219 (+) 1434 WP_048654749.1 6-phospho-beta-glucosidase -
  RFN60_RS01975 (RFN60_01975) nin/comJ 370256..370654 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  RFN60_RS01980 (RFN60_01980) nucA/comI 370681..371130 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  RFN60_RS01985 (RFN60_01985) tlpC 371298..373019 (-) 1722 WP_309520530.1 methyl-accepting chemotaxis protein TlpC -
  RFN60_RS01990 (RFN60_01990) hxlB 373130..373687 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  RFN60_RS01995 (RFN60_01995) hxlA 373693..374325 (-) 633 WP_032722903.1 3-hexulose-6-phosphate synthase -
  RFN60_RS02000 (RFN60_02000) hxlR 374558..374920 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=875540 RFN60_RS01980 WP_003234608.1 370681..371130(-) (nucA/comI) [Bacillus subtilis strain CP38]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=875540 RFN60_RS01980 WP_003234608.1 370681..371130(-) (nucA/comI) [Bacillus subtilis strain CP38]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98