NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	RFF16_RS05885	Genome accession	NZ_CP133642
Coordinates	1276946..1277380 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain Past3
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1271946..1282380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFF16_RS05855 (RFF16_05850)	-	1272510..1272779 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RFF16_RS05860 (RFF16_05855)	yacG	1272779..1272982 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RFF16_RS05865 (RFF16_05860)	coaE	1272993..1273613 (-)	621	WP_010906475.1	dephospho-CoA kinase	-
RFF16_RS05870 (RFF16_05865)	-	1273628..1274350 (-)	723	WP_010906474.1	prepilin peptidase	-
RFF16_RS05875 (RFF16_05870)	-	1274350..1275561 (-)	1212	WP_005722560.1	type II secretion system F family protein	-
RFF16_RS05880 (RFF16_05875)	pilB	1275561..1276946 (-)	1386	WP_010906472.1	GspE/PulE family protein	Machinery gene
RFF16_RS05885 (RFF16_05880)	pilA	1276946..1277380 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RFF16_RS05890 (RFF16_05885)	ampD	1277540..1278106 (+)	567	WP_005751074.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RFF16_RS05895 (RFF16_05890)	rppH	1278691..1279287 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RFF16_RS05900 (RFF16_05895)	-	1279290..1280084 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RFF16_RS05905 (RFF16_05900)	lgt	1280094..1280906 (+)	813	WP_010906471.1	prolipoprotein diacylglyceryl transferase	-
RFF16_RS05910 (RFF16_05905)	-	1280903..1281754 (+)	852	WP_010906470.1	thymidylate synthase	-
RFF16_RS05915 (RFF16_05910)	tadA	1281769..1282269 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=875440 RFF16_RS05885 WP_005741096.1 1276946..1277380(-) (pilA) [Pasteurella multocida strain Past3]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=875440 RFF16_RS05885 WP_005741096.1 1276946..1277380(-) (pilA) [Pasteurella multocida strain Past3]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0875440