Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RFF20_RS06210 Genome accession   NZ_CP133639
Coordinates   1336741..1337286 (-) Length   181 a.a.
NCBI ID   WP_083004641.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1331741..1342286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF20_RS06190 (RFF20_06190) - 1332604..1333215 (-) 612 WP_083004649.1 YfgM family protein -
  RFF20_RS06195 (RFF20_06195) hisS 1333234..1334505 (-) 1272 WP_104227651.1 histidine--tRNA ligase -
  RFF20_RS06200 (RFF20_06200) ispG 1334535..1335638 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RFF20_RS06205 (RFF20_06205) - 1335665..1336618 (-) 954 WP_083004644.1 helix-turn-helix domain-containing protein -
  RFF20_RS06210 (RFF20_06210) pilF/pilF2 1336741..1337286 (-) 546 WP_083004641.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RFF20_RS06215 (RFF20_06215) - 1337326..1338510 (-) 1185 WP_083004640.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RFF20_RS06220 (RFF20_06220) - 1338689..1339942 (-) 1254 WP_005725114.1 AI-2E family transporter -
  RFF20_RS06225 (RFF20_06225) glnB 1339943..1340281 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RFF20_RS06230 (RFF20_06230) mog 1340326..1340904 (-) 579 WP_059245742.1 molybdopterin adenylyltransferase -
  RFF20_RS06235 (RFF20_06235) - 1340930..1341859 (-) 930 WP_059245741.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20819.37 Da        Isoelectric Point: 7.9056

>NTDB_id=875420 RFF20_RS06210 WP_083004641.1 1336741..1337286(-) (pilF/pilF2) [Pasteurella multocida strain Past6]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=875420 RFF20_RS06210 WP_083004641.1 1336741..1337286(-) (pilF/pilF2) [Pasteurella multocida strain Past6]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459