Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RFF39_RS06730 Genome accession   NZ_CP133634
Coordinates   1433383..1433928 (-) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1428383..1438928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF39_RS06705 (RFF39_06705) - 1428594..1429031 (-) 438 WP_323998403.1 isoprenylcysteine carboxylmethyltransferase family protein -
  RFF39_RS06710 (RFF39_06710) - 1429243..1429854 (-) 612 WP_046333474.1 YfgM family protein -
  RFF39_RS06715 (RFF39_06715) hisS 1429873..1431144 (-) 1272 WP_323997313.1 histidine--tRNA ligase -
  RFF39_RS06720 (RFF39_06720) ispG 1431174..1432277 (-) 1104 WP_323997314.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RFF39_RS06725 (RFF39_06725) - 1432304..1433260 (-) 957 WP_014391405.1 helix-turn-helix domain-containing protein -
  RFF39_RS06730 (RFF39_06730) pilF/pilF2 1433383..1433928 (-) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RFF39_RS06735 (RFF39_06735) - 1433967..1435151 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RFF39_RS06740 (RFF39_06740) - 1435331..1436584 (-) 1254 WP_014391407.1 AI-2E family transporter -
  RFF39_RS06745 (RFF39_06745) glnB 1436585..1436923 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RFF39_RS06750 (RFF39_06750) mog 1436968..1437546 (-) 579 WP_170385505.1 molybdopterin adenylyltransferase -
  RFF39_RS06755 (RFF39_06755) - 1437572..1438501 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=875351 RFF39_RS06730 WP_014391406.1 1433383..1433928(-) (pilF/pilF2) [Pasteurella multocida strain Past33]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=875351 RFF39_RS06730 WP_014391406.1 1433383..1433928(-) (pilF/pilF2) [Pasteurella multocida strain Past33]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47