Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RFF25_RS06415 Genome accession   NZ_CP133633
Coordinates   1383612..1384157 (-) Length   181 a.a.
NCBI ID   WP_083004641.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1378612..1389157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF25_RS06395 (RFF25_06390) - 1379475..1380086 (-) 612 WP_083004649.1 YfgM family protein -
  RFF25_RS06400 (RFF25_06395) hisS 1380105..1381376 (-) 1272 WP_083004646.1 histidine--tRNA ligase -
  RFF25_RS06405 (RFF25_06400) ispG 1381406..1382509 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RFF25_RS06410 (RFF25_06405) - 1382536..1383489 (-) 954 WP_083004644.1 helix-turn-helix domain-containing protein -
  RFF25_RS06415 (RFF25_06410) pilF/pilF2 1383612..1384157 (-) 546 WP_083004641.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RFF25_RS06420 (RFF25_06415) - 1384197..1385381 (-) 1185 WP_083004640.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RFF25_RS06425 (RFF25_06420) - 1385560..1386813 (-) 1254 WP_005725114.1 AI-2E family transporter -
  RFF25_RS06430 (RFF25_06425) glnB 1386814..1387152 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RFF25_RS06435 (RFF25_06430) mog 1387197..1387775 (-) 579 WP_249984491.1 molybdopterin adenylyltransferase -
  RFF25_RS06440 (RFF25_06435) - 1387802..1388731 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20819.37 Da        Isoelectric Point: 7.9056

>NTDB_id=875329 RFF25_RS06415 WP_083004641.1 1383612..1384157(-) (pilF/pilF2) [Pasteurella multocida strain Pm1618]
MTRVFQLGIASIFSLFLSACVSQEGHKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=875329 RFF25_RS06415 WP_083004641.1 1383612..1384157(-) (pilF/pilF2) [Pasteurella multocida strain Pm1618]
ATGACGCGCGTTTTTCAATTAGGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTCACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGTGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACACTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459