Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RFF30_RS06325 Genome accession   NZ_CP133632
Coordinates   1383551..1384096 (-) Length   181 a.a.
NCBI ID   WP_046333479.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1621     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1378551..1389096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF30_RS06300 (RFF30_06300) - 1378761..1379198 (-) 438 WP_010907449.1 methyltransferase family protein -
  RFF30_RS06305 (RFF30_06305) - 1379411..1380022 (-) 612 WP_071522791.1 YfgM family protein -
  RFF30_RS06310 (RFF30_06310) hisS 1380041..1381312 (-) 1272 WP_324006946.1 histidine--tRNA ligase -
  RFF30_RS06315 (RFF30_06315) ispG 1381342..1382445 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RFF30_RS06320 (RFF30_06320) - 1382472..1383428 (-) 957 WP_324006947.1 RodZ family helix-turn-helix domain-containing protein -
  RFF30_RS06325 (RFF30_06325) pilF/pilF2 1383551..1384096 (-) 546 WP_046333479.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RFF30_RS06330 (RFF30_06330) - 1384136..1385320 (-) 1185 WP_061406244.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RFF30_RS06335 (RFF30_06335) - 1385500..1386753 (-) 1254 WP_005755688.1 AI-2E family transporter -
  RFF30_RS06340 (RFF30_06340) glnB 1386754..1387092 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RFF30_RS06345 (RFF30_06345) mog 1387137..1387715 (-) 579 WP_324006948.1 molybdopterin adenylyltransferase -
  RFF30_RS06350 (RFF30_06350) - 1387741..1388670 (-) 930 WP_324006949.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20829.28 Da        Isoelectric Point: 7.8917

>NTDB_id=875306 RFF30_RS06325 WP_046333479.1 1383551..1384096(-) (pilF/pilF2) [Pasteurella multocida strain Pm1621]
MNRTFRFGIASIFSLFLSACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=875306 RFF30_RS06325 WP_046333479.1 1383551..1384096(-) (pilF/pilF2) [Pasteurella multocida strain Pm1621]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459