Detailed information NTG0875129

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RE735_RS14270
• Genome accession: NZ_CP133585
• Coordinates: 2654395..2658099 (-)
• Length: 1234 a.a.
• NCBI ID: WP_309173532.1
• Uniprot ID: -
• Organism: Bacillus aerius strain NEB1378
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2649395..2663099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RE735_RS14255 (RE735_14255)	-	2649485..2649784 (-)	300	WP_309173529.1	HNH endonuclease	-
RE735_RS14260 (RE735_14260)	-	2649802..2653197 (-)	3396	WP_309173530.1	SMC family ATPase	-
RE735_RS14265 (RE735_14265)	sbcD	2653194..2654369 (-)	1176	WP_309173531.1	exonuclease subunit SbcD	-
RE735_RS14270 (RE735_14270)	addA	2654395..2658099 (-)	3705	WP_309173532.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RE735_RS14275 (RE735_14275)	addB	2658077..2661586 (-)	3510	WP_309173533.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RE735_RS14280 (RE735_14280)	-	2661754..2662200 (+)	447	WP_007499129.1	ferritin-like domain-containing protein	-
RE735_RS14285 (RE735_14285)	-	2662392..2662802 (-)	411	WP_309173534.1	DoxX family protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142711.87 Da
• Isoelectric Point: 5.2829

>NTDB_id=875129 RE735_RS14270 WP_309173532.1 2654395..2658099(-) (addA) [Bacillus aerius strain NEB1378]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTWPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPTINFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPGQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSKETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHDTESAEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQRLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVLTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGEDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQASDL
LKEDISLAKQKQDEVVQALMEGMPVQGYDDYEEEVKERLLWRYPYLAASQVGTKQSVSEIKRMKEIQDEHSVPSSMRKTH
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIHLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLKEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=875129 RE735_RS14270 WP_309173532.1 2654395..2658099(-) (addA) [Bacillus aerius strain NEB1378]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGTTGATTCGAAAAATGACTTGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGATCACTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCTTTTTGTTTGCAAGTTCTCCGTACTTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCACATCCAGCGCCCGAACAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGGACACTTTATGAACTTTTGCCAACTATCAATTTTAAAAGACTAAAGACGTGCAAAG
GGGACGAGTATGACCCAGCTTTGTTAGAAAAGGCAACAGATGCACGTAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGGTCAGCATTTAAAAAGCTTGGCGGAAATGAAGCCAGTTGTAGAAACACTTGTGGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAAAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCGGATCTAGAGCATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCCAAATACTATCAACAGCAA
TTTGAAGAAGTACTTGTGGATGAATACCAGGATACAAATCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGGGA
ACATCCTGCAGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGACAGCAAGGAAACCGGTAAAAGAATTGATTTAAATAAAAACTTCCGC
AGCCGGGCTGATGTTTTAGACAGTACCAATTTTCTGTTCAAACAGCTGATGGGAGAAACGGTTGGAGAAATTGAATATGA
CGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGCTGAAGAACGAGAAGAGCTAGAGACGGTACAATTTGAGGCAAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGAGGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGCTCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCATCAGGCTATTTCGAAGCGACAGAGGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCGGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCGCTGATTAGAACAAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACATGCAGAAAACTGG
AGCGATTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAATCTTCGAGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGCTTACTGCAGGACTTGGCAGGAACTTTAATATGATGGATCTCAATCAGTCCTACTTGCTTGA
TAAAGAACTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAATTA
TTTTTAGTCGGTTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCTGCAAACGGAGAGGACTGGCTCCT
TCCTGATTTTGAACGTTACCAAGCGAGAACATACTTAGATTTTATTGGTCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTCTTTTAGAAGAATCGAGAGAGGTCGTTCTCTCTCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTAAAGGAAGACATATCACTTGCAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTATGCCTGTTCAAGG
ATATGACGATTATGAGGAAGAAGTAAAAGAAAGGCTTTTATGGCGATATCCATACTTAGCGGCCTCACAAGTAGGGACAA
AACAGTCAGTTTCTGAGATCAAAAGAATGAAAGAAATACAGGATGAACACAGTGTGCCTTCTTCCATGCGAAAAACTCAT
GCGACACTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCATCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATTCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAGCTGCGTAAAGCGGATTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAAAGGAAGCTGAAGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTTGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.452	100	0.668