Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RE735_RS14275 Genome accession   NZ_CP133585
Coordinates   2658077..2661586 (-) Length   1169 a.a.
NCBI ID   WP_309173533.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2653077..2666586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS14265 (RE735_14265) sbcD 2653194..2654369 (-) 1176 WP_309173531.1 exonuclease subunit SbcD -
  RE735_RS14270 (RE735_14270) addA 2654395..2658099 (-) 3705 WP_309173532.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RE735_RS14275 (RE735_14275) addB 2658077..2661586 (-) 3510 WP_309173533.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RE735_RS14280 (RE735_14280) - 2661754..2662200 (+) 447 WP_007499129.1 ferritin-like domain-containing protein -
  RE735_RS14285 (RE735_14285) - 2662392..2662802 (-) 411 WP_309173534.1 DoxX family protein -
  RE735_RS14290 (RE735_14290) - 2662859..2663698 (-) 840 WP_309173535.1 M56 family metallopeptidase -
  RE735_RS14295 (RE735_14295) - 2663691..2664113 (-) 423 WP_309173536.1 BlaI/MecI/CopY family transcriptional regulator -
  RE735_RS14300 (RE735_14300) - 2664369..2665511 (+) 1143 WP_309175511.1 AbrB family transcriptional regulator -
  RE735_RS14305 (RE735_14305) lepB 2665548..2666060 (-) 513 WP_309173537.1 signal peptidase I -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134909.99 Da        Isoelectric Point: 5.3191

>NTDB_id=875130 RE735_RS14275 WP_309173533.1 2658077..2661586(-) (addB) [Bacillus aerius strain NEB1378]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANHQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRRAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSELYQLAKELGAAMSENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHFTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARRVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARFEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYLSYSASDTEGRSILPSTLIKYCEELMPNHQQACYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNRDVSKKIMSSLFFTNRAKRLNPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRQAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMKATPAGILYFHIHDPLIQAPIPLAEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=875130 RE735_RS14275 WP_309173533.1 2658077..2661586(-) (addB) [Bacillus aerius strain NEB1378]
GTGGAGATTCAGTTTTTAGCAGGTAGATCGGGGAGTGGAAAAACGACTGCAATTTTAGAAGAAATCAAAGAACAACTTCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCATCAATTTGTCACAAGCACAGGGATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGGTTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCAGAGGATATTGAAAAAATCTCGGCATCCAGTATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTACAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAAGGGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCAGAAAAAATAACGGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCCAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCAGAGCTTTATCAGTTGGCTAA
AGAGTTGGGAGCGGCTATGTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACTTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTGGATTTAGTAAGAGAGAAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACGCGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGTTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCCAAAAACAAAGCGAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTATGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGCTTTCATTATCGCCGCT
TTCAATCATTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAGGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGTTTGAGGCAGAAGAAGCAGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTATGGGATGCTGTCATTCAATTAATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCCTTATTTCAACAGATGATGGATACAGGACTGTCTTCTCTCAAGTTCGCCTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGAGTTA
TACCTGCGCGGCCCTCTGATGAAAGTGTATTGTCTGAGGACGACCGGGAATGGTTAAAACGTGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATTTATCAAGCGCTGTCTAGTCCATCGCACTACTTGTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTATTTTGCCTTCGACCCTCATCAAGTATTGCGAGGAACTGATGCCAA
ACCATCAGCAAGCCTGCTATGTGTTAGATCCAGAGCAGCTGGATGATCAGGAGCAGTTAAAATTTGTAACAAACGAGCAT
GTCTCGTTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACCGCGACGTATCAAAAAAAATCATGTCCAGCTTGTTCTTTACCAATAGAGCGAAGC
GTTTAAATCCGAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGCTAAAACTAAAAGATCGACAATTTTACAAATTAGAAGCACCTGATATCGG
TCAGCTCTTTCACTCGGCTTTAAAATTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAATTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCAGGCAATTGAACAGCTGGCTCCACGTCTGCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTTCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCTTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGAAGGCGACACCGGCAGGAATTTTATATTTTCATATTCATGACC
CGCTGATTCAAGCCCCTATTCCGCTCGCAGAGGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAAGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAAGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAGATAAATCCATATAAATTAAAGGATCAAACCCCT
TGCCGCTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACATCTGA
GAAGGATGATGTTATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.322

99.316

0.659