Detailed information NTG0874833

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RE438_RS05830
• Genome accession: NZ_CP133557
• Coordinates: 1125700..1129425 (+)
• Length: 1241 a.a.
• NCBI ID: WP_048559588.1
• Uniprot ID: A0A2A7BS66
• Organism: Bacillus wiedmannii strain EPS29
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1120700..1134425

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RE438_RS05815 (RE438_05785)	-	1120717..1121310 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
RE438_RS05820 (RE438_05790)	lepB	1121508..1122071 (+)	564	WP_000751908.1	signal peptidase I	-
RE438_RS05825 (RE438_05795)	addB	1122188..1125703 (+)	3516	WP_048559589.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RE438_RS05830 (RE438_05800)	addA	1125700..1129425 (+)	3726	WP_048559588.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RE438_RS05835 (RE438_05805)	-	1129438..1129725 (+)	288	WP_048559587.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
RE438_RS05840 (RE438_05810)	gerPF	1129804..1130019 (-)	216	WP_001141570.1	spore germination protein GerPF	-
RE438_RS05845 (RE438_05815)	-	1130062..1130448 (-)	387	WP_000902339.1	spore germination protein GerPE	-
RE438_RS05850 (RE438_05820)	gerPD	1130464..1130658 (-)	195	WP_001102341.1	spore germination protein GerPD	-
RE438_RS05855 (RE438_05825)	gerPC	1130665..1131279 (-)	615	WP_001070745.1	spore germination protein GerPC	-
RE438_RS05860 (RE438_05830)	gerPB	1131348..1131554 (-)	207	WP_048559586.1	spore germination protein GerPB	-
RE438_RS05865 (RE438_05835)	gerPA	1131569..1131790 (-)	222	WP_001111187.1	spore germination protein GerPA	-
RE438_RS05870 (RE438_05840)	-	1131886..1132065 (-)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
RE438_RS05875 (RE438_05845)	-	1132312..1133211 (+)	900	WP_048559585.1	fumarylacetoacetate hydrolase family protein	-
RE438_RS05880 (RE438_05850)	-	1133246..1133530 (-)	285	WP_000926860.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142595.78 Da
• Isoelectric Point: 4.8665

>NTDB_id=874833 RE438_RS05830 WP_048559588.1 1125700..1129425(+) (addA) [Bacillus wiedmannii strain EPS29]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAACESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLQWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVHNRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKQPITEEVIREQIAGMVNKELLTFEQAEEVAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=874833 RE438_RS05830 WP_048559588.1 1125700..1129425(+) (addA) [Bacillus wiedmannii strain EPS29]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGCTAGAAAAAGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACAATCCATTCATTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCAGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTTGTGAGTCGTGGACAAGTGTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAACGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGACTATTCTTAGGAAAGTATAAACGTTTTACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTACGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCGGAAGTAGAAAAAGCACAACTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTGCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGACTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAGGAAGAACAAGAAT
TACATGATAAATTACAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCTGGAGCGTCTTGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGCATTCCGGGTGAAATTTATGGATATGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGTTAGAAAGTGAACGAAAAGAAGAAGTGCATAACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTGACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCACCAATTAAAACGCGTCCTCGTTTTATGGAGAAAAAAGGATTAACGTATGCTGAAAGAGG
TACGGCAGTTCACGCTGTTATGCAGCATGTTGATTTGAAGCAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCCG
GAATGGTAAATAAGGAATTATTAACATTCGAACAAGCTGAAGAAGTAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAGAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAAACGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATTGATTTTAAAACGGATACGATTGAAGGAAAGTTCCCAGGTGGATTCGATCAAGCAAAACCAATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

3D structure

• AlphaFold DB: A0A2A7BS66

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534