Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RE438_RS05825 Genome accession   NZ_CP133557
Coordinates   1122188..1125703 (+) Length   1171 a.a.
NCBI ID   WP_048559589.1    Uniprot ID   -
Organism   Bacillus wiedmannii strain EPS29     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1117188..1130703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE438_RS05795 (RE438_05765) - 1117656..1118447 (-) 792 WP_048559591.1 phosphotransferase -
  RE438_RS05800 (RE438_05770) cspA 1118822..1119025 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  RE438_RS05805 (RE438_05775) - 1119278..1119463 (-) 186 WP_000391438.1 hypothetical protein -
  RE438_RS05810 (RE438_05780) - 1119747..1120328 (+) 582 WP_048559590.1 competence protein ComK -
  RE438_RS05815 (RE438_05785) - 1120717..1121310 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  RE438_RS05820 (RE438_05790) lepB 1121508..1122071 (+) 564 WP_000751908.1 signal peptidase I -
  RE438_RS05825 (RE438_05795) addB 1122188..1125703 (+) 3516 WP_048559589.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RE438_RS05830 (RE438_05800) addA 1125700..1129425 (+) 3726 WP_048559588.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RE438_RS05835 (RE438_05805) - 1129438..1129725 (+) 288 WP_048559587.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  RE438_RS05840 (RE438_05810) gerPF 1129804..1130019 (-) 216 WP_001141570.1 spore germination protein GerPF -
  RE438_RS05845 (RE438_05815) - 1130062..1130448 (-) 387 WP_000902339.1 spore germination protein GerPE -
  RE438_RS05850 (RE438_05820) gerPD 1130464..1130658 (-) 195 WP_001102341.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134381.49 Da        Isoelectric Point: 5.1786

>NTDB_id=874832 RE438_RS05825 WP_048559589.1 1122188..1125703(+) (addB) [Bacillus wiedmannii strain EPS29]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKEGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEILRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRVRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=874832 RE438_RS05825 WP_048559589.1 1122188..1125703(+) (addB) [Bacillus wiedmannii strain EPS29]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
GCAACGCCCAAGAGGGAAAACAATTTTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATCGGGA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTCGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTGCTTCGTAAAATTGTTGAGTCTCGTAAAGAGGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGACGCACATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGTTAGTCGAAAAGCTTCCACAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCGT
TCTCACCGCAAGAACTAGAAATTTTAAGACAGCTTATGATTTGCGGCGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGCTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGGGTTGCGCGTGAAATTCGTAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCGAAAAAACTAGAAGCATTACGTGTTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGATGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCCTTAAGAGAGCGTGATATTTTCAAATTGGATGC
GCCAGATATCGGGGAACTATTCCATGCGGCGCTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTAAAGATGGAAGTAGTTGGTCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAACGGA
ACGTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAAGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGACGCTGATGTTGTGCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGCAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATTGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.319

100

0.494