Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   WJ038_RS12830 Genome accession   NZ_CP149505
Coordinates   2773141..2773734 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain EB101     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2768141..2778734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ038_RS12810 (WJ038_12860) aroB 2769017..2770117 (-) 1101 WP_005496576.1 3-dehydroquinate synthase -
  WJ038_RS12815 (WJ038_12865) aroK 2770149..2770667 (-) 519 WP_005381319.1 shikimate kinase AroK -
  WJ038_RS12820 (WJ038_12870) pilQ 2770856..2772604 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  WJ038_RS12825 (WJ038_12875) pilP 2772636..2773151 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  WJ038_RS12830 (WJ038_12880) pilO 2773141..2773734 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  WJ038_RS12835 (WJ038_12885) pilN 2773727..2774305 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  WJ038_RS12840 (WJ038_12890) pilM 2774289..2775305 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  WJ038_RS12845 (WJ038_12895) - 2775448..2778018 (+) 2571 WP_029804285.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=874757 WJ038_RS12830 WP_005480482.1 2773141..2773734(-) (pilO) [Vibrio parahaemolyticus strain EB101]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=874757 WJ038_RS12830 WP_005480482.1 2773141..2773734(-) (pilO) [Vibrio parahaemolyticus strain EB101]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726