Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   RDV54_RS06945 Genome accession   NZ_CP133471
Coordinates   1462517..1463815 (-) Length   432 a.a.
NCBI ID   WP_000867722.1    Uniprot ID   A0AAX2L3B0
Organism   Streptococcus mitis strain LMG 14557     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1457517..1468815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV54_RS06925 (RDV54_06925) rplI 1458672..1459124 (-) 453 WP_000864218.1 50S ribosomal protein L9 -
  RDV54_RS06930 (RDV54_06930) - 1459121..1461094 (-) 1974 WP_000715136.1 DHH family phosphoesterase -
  RDV54_RS06935 (RDV54_06935) hpf 1461230..1461778 (-) 549 WP_000599102.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RDV54_RS06940 (RDV54_06940) comFC/cflB 1461858..1462520 (-) 663 WP_000649953.1 ComF family protein Machinery gene
  RDV54_RS06945 (RDV54_06945) comFA/cflA 1462517..1463815 (-) 1299 WP_000867722.1 DEAD/DEAH box helicase Machinery gene
  RDV54_RS06950 (RDV54_06950) - 1463872..1464507 (+) 636 WP_000395748.1 YigZ family protein -
  RDV54_RS06955 (RDV54_06955) - 1464523..1464966 (+) 444 WP_000863906.1 PH domain-containing protein -
  RDV54_RS06960 (RDV54_06960) cysK 1465063..1465989 (+) 927 WP_000029880.1 cysteine synthase A -
  RDV54_RS06965 (RDV54_06965) tsf 1466111..1467151 (-) 1041 WP_000808076.1 translation elongation factor Ts -
  RDV54_RS06970 (RDV54_06970) rpsB 1467230..1468009 (-) 780 WP_000268459.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49667.61 Da        Isoelectric Point: 8.6690

>NTDB_id=874503 RDV54_RS06945 WP_000867722.1 1462517..1463815(-) (comFA/cflA) [Streptococcus mitis strain LMG 14557]
MKVNPNYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGTYYCRECLLMKRVRSDQSLYYFPQ
EDFPKQDVLKWRGQLTPFQDKVSQGLLQAVDKQEPSLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQKDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKSYIEKQRKTGYPLLIFASEIKKGEQLKEI
IQEQFPNEKIGFVSSITENRLEQVQAFRDRELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNTSIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=874503 RDV54_RS06945 WP_000867722.1 1462517..1463815(-) (comFA/cflA) [Streptococcus mitis strain LMG 14557]
ATGAAAGTAAATCCAAATTATCTCGGTCGCTTGTTTACTGAGAATGAATTAACAGAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTACTTACTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAATCTTTATACTATTTCCCACAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGCGTGGTCAATTAACCCCTTTTCAAGATAAGGTATCACAGGGACTGCT
TCAAGCGGTAGACAAGCAAGAGCCAAGCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGACGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAAGGATTTTGCTTGTGAGATAGCCCTCCTACATGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTACTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCTATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAGTCGGTTGTCACCAAAGCTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAGATTAAGAAAGGGGAACAGTTAAAAGAAATC
ATACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATAGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGAGTTACCTTCCCTTGTGTTGACGTTTTCG
TAGTAGAGGCCAATCATCGTCTGTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGACGGGTTAAATACTTCAATCAAAAAGGCAATTAAGGAAATTCAACAGATGAA
TAAGGAGGCGGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

100

100

1
  comFA/cflA Streptococcus mitis SK321

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae Rx1

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae D39

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae R6

93.519

100

0.935
  comFA/cflA Streptococcus pneumoniae TIGR4

93.287

100

0.933
  comFA Lactococcus lactis subsp. cremoris KW2

50.117

99.306

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.515

99.769

0.384