Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   K6K00_RS20030 Genome accession   NZ_AP024628
Coordinates   3844001..3844120 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain BEST3145     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3839001..3849120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6K00_RS20010 (BsBEST3145_38920) albG 3839165..3839866 (+) 702 WP_014481222.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  K6K00_RS20015 (BsBEST3145_38930) ywhL 3839872..3841248 (-) 1377 WP_014481223.1 YncE family protein -
  K6K00_RS20020 (BsBEST3145_38940) ywhK 3841287..3842642 (-) 1356 WP_014481224.1 YncE family protein -
  K6K00_RS22145 - 3842639..3842869 (-) 231 Protein_3924 hypothetical protein -
  K6K00_RS20025 (BsBEST3145_38950) rapF 3842872..3844017 (+) 1146 WP_014481226.1 response regulator aspartate phosphatase RapF Regulator
  K6K00_RS20030 (BsBEST3145_38960) phrF 3844001..3844120 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  K6K00_RS20035 (BsBEST3145_38970) - 3844218..3844645 (+) 428 Protein_3927 YbaK/EbsC family protein -
  K6K00_RS20040 (BsBEST3145_38980) speB 3844710..3845582 (-) 873 WP_003227545.1 agmatinase -
  K6K00_RS20045 (BsBEST3145_38990) speE 3845643..3846473 (-) 831 WP_003227543.1 spermidine synthase -
  K6K00_RS20050 (BsBEST3145_39000) pbpG 3846675..3848750 (+) 2076 WP_014481227.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=87407 K6K00_RS20030 WP_009968329.1 3844001..3844120(+) (phrF) [Bacillus subtilis strain BEST3145]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=87407 K6K00_RS20030 WP_009968329.1 3844001..3844120(+) (phrF) [Bacillus subtilis strain BEST3145]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487

Multiple sequence alignment