Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   K6J86_RS19965 Genome accession   NZ_AP024627
Coordinates   3821234..3821353 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain BEST3136     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3816234..3826353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J86_RS19945 (BsBEST3136_38550) albG 3816398..3817099 (+) 702 WP_014481222.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  K6J86_RS19950 (BsBEST3136_38560) ywhL 3817105..3818481 (-) 1377 WP_014481223.1 YncE family protein -
  K6J86_RS19955 (BsBEST3136_38570) ywhK 3818520..3819875 (-) 1356 WP_014481224.1 YncE family protein -
  K6J86_RS22060 - 3819872..3820102 (-) 231 Protein_3893 hypothetical protein -
  K6J86_RS19960 (BsBEST3136_38580) rapF 3820105..3821250 (+) 1146 WP_014481226.1 response regulator aspartate phosphatase RapF Regulator
  K6J86_RS19965 (BsBEST3136_38590) phrF 3821234..3821353 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  K6J86_RS19970 (BsBEST3136_38600) - 3821451..3821878 (+) 428 Protein_3896 YbaK/EbsC family protein -
  K6J86_RS19975 (BsBEST3136_38610) speB 3821943..3822815 (-) 873 WP_003227545.1 agmatinase -
  K6J86_RS19980 (BsBEST3136_38620) speE 3822876..3823706 (-) 831 WP_003227543.1 spermidine synthase -
  K6J86_RS19985 (BsBEST3136_38630) pbpG 3823908..3825983 (+) 2076 WP_014481227.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=87324 K6J86_RS19965 WP_009968329.1 3821234..3821353(+) (phrF) [Bacillus subtilis strain BEST3136]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=87324 K6J86_RS19965 WP_009968329.1 3821234..3821353(+) (phrF) [Bacillus subtilis strain BEST3136]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487


Multiple sequence alignment