Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABJ65_RS03765 Genome accession   NZ_AP027553
Coordinates   772908..773717 (+) Length   269 a.a.
NCBI ID   WP_001333829.1    Uniprot ID   Q46836
Organism   Escherichia coli strain LR-58-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 767908..778717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ65_RS03755 (VEE07_07230) glcA 769706..771388 (+) 1683 WP_000259302.1 glycolate permease GlcA -
  AABJ65_RS03760 (VEE07_07250) - 771873..772724 (+) 852 Protein_737 Accessory colonization factor AcfD -
  AABJ65_RS03765 (VEE07_07260) pilD 772908..773717 (+) 810 WP_001333829.1 prepilin peptidase PppA Machinery gene
  AABJ65_RS03770 (VEE07_07270) gspS2 773783..774193 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  AABJ65_RS03775 (VEE07_07280) gspC 774211..775170 (+) 960 WP_000135076.1 type II secretion system protein GspC -
  AABJ65_RS03780 (VEE07_07290) - 775200..775970 (+) 771 Protein_741 hypothetical protein -
  AABJ65_RS03785 (VEE07_07300) gspK 775967..776536 (+) 570 Protein_742 type II secretion system minor pseudopilin GspK -
  AABJ65_RS03790 (VEE07_07310) gspL 776533..777711 (+) 1179 WP_000094991.1 type II secretion system protein GspL -
  AABJ65_RS03795 (VEE07_07320) yghD 777713..778249 (+) 537 WP_000942785.1 GspM family type II secretion system protein YghD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29466.06 Da        Isoelectric Point: 8.3829

>NTDB_id=87322 AABJ65_RS03765 WP_001333829.1 772908..773717(+) (pilD) [Escherichia coli strain LR-58-1]
MLFDVFQQYPTAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRIRDNIP
LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=87322 AABJ65_RS03765 WP_001333829.1 772908..773717(+) (pilD) [Escherichia coli strain LR-58-1]
ATGCTTTTTGATGTTTTTCAGCAATACCCCACGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCAATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGTTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACAGCACTTGCTTTTTTGCTGGCGAGTCTGGTCTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATCGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGCTCACGCTACAAGATGCAGTCACCGGCGTCCTGGTGGGATTTATCAC
TTTTTACTCTCTGCGTTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCCG
CGTTAGGTGGCTGGGTGGGGGCATTGTCGCTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q46836

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.944

99.257

0.446
  pilD Vibrio campbellii strain DS40M4

42.435

100

0.428
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372

Multiple sequence alignment