Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   RAM80_RS13045 Genome accession   NZ_CP133165
Coordinates   2811024..2812616 (+) Length   530 a.a.
NCBI ID   WP_416426946.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 2806024..2817616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS13025 (RAM80_13005) pgeF 2807648..2808388 (-) 741 WP_416426942.1 peptidoglycan editing factor PgeF -
  RAM80_RS13030 (RAM80_13010) rluD 2808385..2809347 (-) 963 WP_416426943.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  RAM80_RS13035 (RAM80_13015) - 2809497..2810522 (+) 1026 WP_416426944.1 outer membrane protein assembly factor BamD -
  RAM80_RS13040 (RAM80_13020) - 2810801..2811034 (+) 234 WP_416426945.1 PP0621 family protein -
  RAM80_RS13045 (RAM80_13025) pilS 2811024..2812616 (+) 1593 WP_416426946.1 sensor histidine kinase Regulator
  RAM80_RS13050 (RAM80_13030) pilR 2812619..2813959 (+) 1341 WP_416426947.1 sigma-54-dependent transcriptional regulator Regulator
  RAM80_RS13055 (RAM80_13035) thiO 2814105..2815208 (-) 1104 WP_416426948.1 glycine oxidase ThiO -
  RAM80_RS13060 (RAM80_13040) - 2815234..2815638 (-) 405 WP_416426949.1 type IV pilin protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58720.99 Da        Isoelectric Point: 8.6202

>NTDB_id=872248 RAM80_RS13045 WP_416426946.1 2811024..2812616(+) (pilS) [Pseudomonas sp. App30]
MSAETFLPGGPQARRLLRLYHLYRLTIGITLILLISSNMDDKLLELANGAWFRTGSWAYLLFNIAAVILLEKLRPPGHVF
ALALLDILMLSGLFYAGGGTGSGIGNLMIASVAMSNLLLRGRNGLLIAALGSIGIIYLTFYLNFLRPNSATNYVQASTLG
VLCFAVALMVQALTRLLRVSESLAEQRASDVTNLEELNALILQRMRTGILVLDTVNRVQLANEGALKLIGQFNLGGQLIT
AYSPELVERLLQWRTNPTLRLRSLKFTPTGPSVQPSFIALRRGEQQQTLVFLEDLSQVAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAGQLLQESENLDPADLRLTEIIKDQSKRMNLIIENVLQLSRRRQAEPEPLDLRAFLERFLADFRTTATPR
QALHLYVTDGPIETRMDPHHLTQVLSNLVENGLRYSAHAHGQAQVWLNLFVDPTNGLPILDVLDDGPGFSADQLQHIFEP
FFTTETKGTGLGLYISRELCESNQAHLDYKPRERGGSCLRITFAHARKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=872248 RAM80_RS13045 WP_416426946.1 2811024..2812616(+) (pilS) [Pseudomonas sp. App30]
GTGAGCGCTGAGACCTTCCTGCCGGGCGGCCCCCAGGCGCGGCGCCTGCTGCGCCTGTACCATCTGTACCGCCTGACCAT
CGGCATCACCCTGATCCTGCTGATTTCCAGCAACATGGATGACAAGCTGCTGGAGCTGGCCAACGGCGCCTGGTTCCGCA
CGGGCAGCTGGGCCTACCTGTTGTTCAACATCGCCGCCGTCATCCTCCTGGAAAAGCTGCGCCCGCCCGGCCACGTGTTC
GCCCTGGCGCTGCTCGACATCCTCATGCTCTCGGGGCTGTTCTACGCCGGTGGCGGCACGGGCAGCGGCATTGGCAACCT
GATGATCGCCTCGGTGGCCATGAGCAACCTGCTGCTGCGCGGGCGCAACGGCCTGCTGATCGCCGCGCTGGGCAGCATCG
GCATCATCTACCTGACCTTCTACCTGAACTTCCTGCGCCCCAACTCCGCCACCAACTACGTGCAGGCCAGCACACTGGGG
GTGCTGTGCTTTGCCGTGGCGCTGATGGTGCAGGCCCTGACGCGGCTGCTGCGGGTCAGCGAGAGCCTGGCCGAGCAGCG
CGCCAGCGACGTCACCAACCTGGAAGAGCTCAACGCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTGGATA
CGGTCAACCGCGTGCAGCTGGCCAACGAAGGCGCGCTCAAGCTGATCGGCCAGTTCAACCTGGGCGGCCAATTGATCACC
GCCTACTCACCGGAGCTGGTGGAGCGCCTGCTACAGTGGCGCACCAACCCTACCCTGCGCCTGCGCAGCCTGAAGTTCAC
GCCCACCGGCCCTTCGGTGCAGCCCAGCTTCATCGCCCTGCGCCGCGGCGAGCAGCAGCAGACCCTGGTGTTCCTCGAAG
ACCTCTCGCAGGTCGCCCAACAGGCCCAGCAACTCAAGCTGGCTGCCCTGGGCCGGCTGACCGCGGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGGGCCATCAGCCACGCCGGCCAGTTGTTGCAGGAGTCGGAAAACCTCGACCCCGCCGACCTGCG
CCTGACCGAGATTATCAAGGACCAGTCCAAGCGCATGAACCTGATCATCGAGAACGTGCTGCAGCTGTCCCGGCGCCGCC
AGGCCGAGCCCGAGCCGCTGGACCTGCGGGCGTTCCTCGAGCGCTTCCTCGCCGACTTCCGCACCACCGCCACGCCGCGC
CAGGCCCTGCACCTGTATGTTACCGACGGGCCGATCGAGACCCGCATGGACCCGCACCACCTGACCCAGGTGCTCAGCAA
CCTGGTGGAAAACGGCCTGCGCTACAGCGCCCACGCCCATGGCCAGGCCCAAGTGTGGCTGAACCTGTTCGTCGACCCGA
CCAACGGCCTGCCGATTCTCGACGTGCTGGATGATGGCCCGGGTTTTTCAGCCGATCAACTGCAACACATCTTCGAACCT
TTCTTCACCACCGAAACCAAAGGAACAGGATTGGGCCTCTACATTTCCCGTGAACTGTGCGAAAGCAACCAGGCGCACCT
AGACTACAAGCCACGTGAACGCGGCGGCAGTTGCCTGCGTATCACGTTCGCCCATGCACGGAAGCTGAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.509

100

0.615