Detailed information NTG0872247

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RAM80_RS12950
• Genome accession: NZ_CP133165
• Coordinates: 2795926..2796795 (+)
• Length: 289 a.a.
• NCBI ID: WP_416426932.1
• Uniprot ID: -
• Organism: Pseudomonas sp. App30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2790926..2801795

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAM80_RS12920 (RAM80_12900)	-	2791297..2791686 (+)	390	WP_416426927.1	hypothetical protein	-
RAM80_RS12930 (RAM80_12910)	-	2791866..2792291 (-)	426	WP_416426928.1	pilin	-
RAM80_RS12935 (RAM80_12915)	-	2792349..2792768 (-)	420	WP_416426929.1	pilin	-
RAM80_RS12940 (RAM80_12920)	pilB	2792997..2794697 (+)	1701	WP_416426930.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RAM80_RS12945 (RAM80_12925)	pilC	2794701..2795924 (+)	1224	WP_416426931.1	type II secretion system F family protein	Machinery gene
RAM80_RS12950 (RAM80_12930)	pilD	2795926..2796795 (+)	870	WP_416426932.1	prepilin peptidase	Machinery gene
RAM80_RS12955 (RAM80_12935)	coaE	2796795..2797418 (+)	624	WP_416426933.1	dephospho-CoA kinase	-
RAM80_RS12960 (RAM80_12940)	yacG	2797415..2797618 (+)	204	WP_416426934.1	DNA gyrase inhibitor YacG	-
RAM80_RS12965 (RAM80_12945)	-	2797748..2798425 (-)	678	WP_416426935.1	energy-coupling factor ABC transporter permease	-
RAM80_RS12970 (RAM80_12950)	-	2798422..2798889 (-)	468	WP_008371544.1	hypothetical protein	-
RAM80_RS12975 (RAM80_12955)	-	2799001..2799630 (+)	630	WP_416426936.1	DUF1780 domain-containing protein	-
RAM80_RS12980 (RAM80_12960)	-	2799806..2799979 (+)	174	WP_416426937.1	DUF3094 domain-containing protein	-
RAM80_RS12985 (RAM80_12965)	-	2800043..2801338 (+)	1296	WP_416426938.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31502.66 Da
• Isoelectric Point: 8.3534

>NTDB_id=872247 RAM80_RS12950 WP_416426932.1 2795926..2796795(+) (pilD) [Pseudomonas sp. App30]
MPLSDYLATSPVAFTLCALILGLVVGSFLNVIIHRLPKMMERDWKAQARDILGLPAEPEGPRYNLMLPASACPHCQHKIR
AWENIPVVSWLALKGQCSQCKAPISKRYPLVELASGLLSAFVAWHFGFGWAAGTMMLLTWGLLAMSMIDADHQLLPDSLV
LPLLWLGLIVNAFGLFATLPDALWGAVAGYLALWLVFWLFKLVTGKEGMGHGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AILGVILLRLRNAAHSTPIPFGPYLAIAGWIALLWGGQITASYMQFAGF

Nucleotide

• Length: 870 bp

>NTDB_id=872247 RAM80_RS12950 WP_416426932.1 2795926..2796795(+) (pilD) [Pseudomonas sp. App30]
ATGCCCCTATCCGATTACCTGGCCACCTCCCCCGTGGCCTTCACCCTCTGCGCCCTGATCCTGGGCCTCGTAGTGGGCAG
TTTCCTCAACGTCATCATCCACCGCCTGCCGAAAATGATGGAGCGCGACTGGAAGGCCCAGGCCCGCGACATCCTCGGCT
TGCCGGCCGAGCCCGAGGGCCCGCGCTACAACCTGATGCTACCTGCCTCGGCCTGCCCGCATTGCCAGCACAAGATCCGC
GCTTGGGAAAACATCCCCGTGGTCAGCTGGCTGGCGCTGAAGGGCCAATGCAGCCAGTGCAAGGCGCCCATCAGCAAGCG
CTACCCGTTGGTGGAGCTGGCCAGTGGCCTGTTGAGCGCGTTCGTTGCCTGGCATTTCGGCTTTGGCTGGGCCGCCGGGA
CGATGATGCTGCTGACCTGGGGCCTGCTGGCCATGAGCATGATCGATGCCGACCATCAGTTGCTGCCGGATTCGCTGGTG
CTGCCGTTGCTATGGCTGGGCTTGATCGTCAATGCGTTCGGCCTGTTTGCCACGCTGCCCGACGCACTGTGGGGCGCGGT
GGCCGGCTACCTGGCGCTGTGGCTGGTGTTCTGGCTATTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCCATGGCGACT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGTGGCTGGCAGGTGTTGCCGCTGACCATCCTGCTGTCGTCGCTGGTGGGC
GCCATTCTCGGGGTGATCCTGCTGCGCCTGCGCAATGCTGCGCACAGCACGCCGATCCCTTTCGGGCCGTATCTGGCCAT
TGCGGGCTGGATCGCATTGCTGTGGGGTGGTCAAATAACCGCTTCCTACATGCAGTTTGCTGGTTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.71	95.502	0.522
pilD	Vibrio campbellii strain DS40M4	52.174	95.502	0.498
pilD	Acinetobacter baumannii D1279779	50.534	97.232	0.491
pilD	Acinetobacter nosocomialis M2	49.11	97.232	0.478
pilD	Neisseria gonorrhoeae MS11	50.741	93.426	0.474