Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   RBI06_RS25515 Genome accession   NZ_CP133093
Coordinates   5492485..5494077 (+) Length   530 a.a.
NCBI ID   WP_033989041.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 002     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5456553..5503599 5492485..5494077 within 0


Gene organization within MGE regions


Location: 5456553..5503599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBI06_RS25315 (RBI06_25315) - 5456553..5456846 (-) 294 WP_015503624.1 IS3 family transposase -
  RBI06_RS25320 (RBI06_25320) - 5456981..5457658 (+) 678 WP_015503625.1 thermostable hemolysin -
  RBI06_RS25325 (RBI06_25325) - 5457648..5459114 (+) 1467 WP_015503626.1 AMP-binding protein -
  RBI06_RS25330 (RBI06_25330) - 5459123..5459803 (+) 681 WP_015503627.1 TenA family transcriptional regulator -
  RBI06_RS25335 (RBI06_25335) - 5459790..5460596 (+) 807 WP_015503628.1 SDR family oxidoreductase -
  RBI06_RS25340 (RBI06_25340) - 5460593..5461234 (+) 642 WP_015503629.1 tetratricopeptide repeat protein -
  RBI06_RS25345 (RBI06_25345) - 5461237..5461899 (+) 663 WP_015503630.1 response regulator transcription factor -
  RBI06_RS25350 (RBI06_25350) - 5461896..5463263 (+) 1368 WP_023111833.1 ATP-binding protein -
  RBI06_RS25355 (RBI06_25355) - 5463659..5464468 (-) 810 WP_015503632.1 diheme cytochrome c -
  RBI06_RS25360 (RBI06_25360) - 5464483..5464896 (-) 414 WP_015503633.1 DUF1924 domain-containing protein -
  RBI06_RS25365 (RBI06_25365) - 5465035..5466546 (+) 1512 WP_023980673.1 2Fe-2S iron-sulfur cluster-binding protein -
  RBI06_RS25370 (RBI06_25370) - 5466761..5466965 (-) 205 Protein_5023 IS5/IS1182 family transposase -
  RBI06_RS25375 (RBI06_25375) - 5467049..5468175 (+) 1127 WP_094867530.1 IS3 family transposase -
  RBI06_RS25380 (RBI06_25380) - 5468578..5469084 (+) 507 WP_031277313.1 chalcone isomerase family protein -
  RBI06_RS25385 (RBI06_25385) - 5469081..5469635 (+) 555 WP_015503637.1 DUF3833 domain-containing protein -
  RBI06_RS25390 (RBI06_25390) - 5469632..5469937 (+) 306 WP_003123379.1 DUF6482 family protein -
  RBI06_RS25395 (RBI06_25395) - 5469934..5471475 (+) 1542 WP_015503638.1 cryptochrome/photolyase family protein -
  RBI06_RS25400 (RBI06_25400) - 5471472..5471627 (+) 156 WP_015503639.1 DUF2256 domain-containing protein -
  RBI06_RS25405 (RBI06_25405) - 5471592..5472083 (-) 492 WP_023111836.1 DUF2878 domain-containing protein -
  RBI06_RS25410 (RBI06_25410) - 5472083..5473351 (-) 1269 WP_015503641.1 cyclopropane-fatty-acyl-phospholipid synthase family protein -
  RBI06_RS25415 (RBI06_25415) - 5473323..5474138 (-) 816 WP_015503642.1 DUF1365 domain-containing protein -
  RBI06_RS25420 (RBI06_25420) - 5474135..5475382 (-) 1248 WP_023980440.1 NAD(P)/FAD-dependent oxidoreductase -
  RBI06_RS25425 (RBI06_25425) - 5475386..5476141 (-) 756 WP_003123384.1 SDR family oxidoreductase -
  RBI06_RS25430 (RBI06_25430) - 5476138..5476560 (-) 423 WP_003123385.1 nuclear transport factor 2 family protein -
  RBI06_RS25435 (RBI06_25435) - 5476657..5477127 (-) 471 WP_003123386.1 PAS domain-containing protein -
  RBI06_RS25440 (RBI06_25440) - 5477255..5478148 (+) 894 WP_003123387.1 MerR family transcriptional regulator -
  RBI06_RS25445 (RBI06_25445) - 5478343..5479713 (+) 1371 WP_023082828.1 IS1182 family transposase -
  RBI06_RS25450 (RBI06_25450) - 5479964..5481475 (-) 1512 WP_033983075.1 IS66 family transposase -
  RBI06_RS25455 (RBI06_25455) tnpB 5481539..5481874 (-) 336 WP_023980255.1 IS66 family insertion sequence element accessory protein TnpB -
  RBI06_RS25460 (RBI06_25460) - 5481871..5482212 (-) 342 WP_023980254.1 transposase -
  RBI06_RS25465 (RBI06_25465) mobH 5482463..5484385 (+) 1923 WP_023111839.1 MobH family relaxase -
  RBI06_RS25470 (RBI06_25470) xerC 5484382..5485662 (+) 1281 WP_015503393.1 tyrosine recombinase XerC -
  RBI06_RS25490 (RBI06_25490) clpB 5486515..5489079 (-) 2565 WP_003094682.1 ATP-dependent chaperone ClpB -
  RBI06_RS25495 (RBI06_25495) pgeF 5489235..5489963 (-) 729 WP_003094684.1 peptidoglycan editing factor PgeF -
  RBI06_RS25500 (RBI06_25500) rluD 5489960..5490922 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  RBI06_RS25505 (RBI06_25505) - 5491068..5492093 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  RBI06_RS25510 (RBI06_25510) - 5492250..5492495 (+) 246 WP_003102591.1 PP0621 family protein -
  RBI06_RS25515 (RBI06_25515) pilS 5492485..5494077 (+) 1593 WP_033989041.1 two-component system sensor histidine kinase PilS Regulator
  RBI06_RS25520 (RBI06_25520) pilR 5494092..5495429 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  RBI06_RS25525 (RBI06_25525) thiO 5495475..5496569 (-) 1095 WP_003141551.1 glycine oxidase ThiO -
  RBI06_RS25530 (RBI06_25530) - 5496709..5497209 (+) 501 WP_016562346.1 GspH/FimT family pseudopilin -
  RBI06_RS25535 (RBI06_25535) - 5497332..5497820 (+) 489 WP_003141552.1 GspH/FimT family pseudopilin -
  RBI06_RS25540 (RBI06_25540) pilV 5497830..5498354 (+) 525 WP_003116261.1 type IV pilus modification protein PilV Machinery gene
  RBI06_RS25545 (RBI06_25545) - 5498363..5499178 (+) 816 WP_016254061.1 PilW family protein -
  RBI06_RS25550 (RBI06_25550) - 5499175..5499780 (+) 606 WP_003116263.1 PilX N-terminal domain-containing pilus assembly protein -
  RBI06_RS25555 (RBI06_25555) - 5499794..5503270 (+) 3477 WP_023100239.1 pilus assembly protein -
  RBI06_RS25560 (RBI06_25560) - 5503273..5503599 (+) 327 WP_016254060.1 PilY2 family type 4a fimbrial biogenesis protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58984.21 Da        Isoelectric Point: 6.9612

>NTDB_id=871852 RBI06_RS25515 WP_033989041.1 5492485..5494077(+) (pilS) [Pseudomonas aeruginosa strain 002]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEKRAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=871852 RBI06_RS25515 WP_033989041.1 5492485..5494077(+) (pilS) [Pseudomonas aeruginosa strain 002]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAAAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.434

100

0.994