Detailed information NTG0871722

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: RBH91_RS06280
• Genome accession: NZ_CP133085
• Coordinates: 1314578..1318270 (+)
• Length: 1230 a.a.
• NCBI ID: WP_307893845.1
• Uniprot ID: -
• Organism: Bacillus swezeyi strain EA208
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1309578..1323270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBH91_RS06270	modB	1310335..1310991 (+)	657	WP_307894685.1	molybdate ABC transporter permease subunit	-
RBH91_RS06275	addB	1311091..1314591 (+)	3501	WP_307893844.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
RBH91_RS06280	addA	1314578..1318270 (+)	3693	WP_307893845.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
RBH91_RS06285	sbcD	1318340..1319521 (+)	1182	WP_307893846.1	exonuclease subunit SbcD	-
RBH91_RS06290	sbcC	1319518..1322910 (+)	3393	WP_307893847.1	exonuclease subunit SbcC	-
RBH91_RS06295	-	1323026..1323244 (-)	219	WP_026586331.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141401.24 Da
• Isoelectric Point: 5.2684

>NTDB_id=871722 RBH91_RS06280 WP_307893845.1 1314578..1318270(+) (addA) [Bacillus swezeyi strain EA208]
MEMSKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITEKENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNRASISTLHSFCLQVVRKYYYLIDIDPAFRIADQTEGELLGDEVLDDLFEEEYKKQDPA
FFELVDRYTTDRHDLDLQYLVKRVYEFSRSHPDPEGWLEKFVDLYDAAQDEKVESLPFYSYIKEDIALILEGVREKLARA
LELAKQPGGPAPRAENFVDDLKQIDRLIQHQDDFSALYELVPAVSFQRAKTCKGDEFEPALIEESTELRNSAKKQLEKLK
SDYFTRTPDQHLASLKEMKPVIHTLVQLVLEYGKRFTAAKKEKAIVDFSDLEHDCLAILTKKNDAGETGPSEAAKYYRLQ
FHEVLVDEYQDTNLVQEAILKLVAKDEPEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTEDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVDYDEQAELKLGASYPPNETTKTELLIIENQGDAADEEAEELETVQLEARAMAMQIRKL
ISEKFQVYDGKTKTTRNIQYRDIVILLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSVLKVIDNAYQDIPL
ASVLRSPVVGLDENELSVIRIQDKKAPFYEAMKAYLASGDRDGSLYEKLRRFEGLLRKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPMLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLYLIG
SAKDKEKLLSKWRTTAAQPEWLLPDFDRFQAKSYLDFIGPALMRHRDMADAGVDSPDDEIRQHPSRFLISWLSGGELQAE
DAKQAGEEKHERLTQIKKGLPIDAAFDYQEEVEERLSWSYPYQDAAKVRTKQSVSEIKRQKEYEDEYGDRSLIRPANEAL
LFRRPSFMMTKGLTAAERGTAMHTVMQHIPLTRAPEKDELSRLLDRLVDKELLTEEQRAAIEEDDILAFFETDIGEKLLG
AHRVEREVPFNMTLPAAEVYPNLQTADEPVLIQGIIDCLFETEDGFYLLDYKTDRIQGKYRNGFEGAEPVLRKRYETQIH
LYARAVETMLKMPLKGRALYFFDGGHVLKF

Nucleotide

• Length: 3693 bp

>NTDB_id=871722 RBH91_RS06280 WP_307893845.1 1314578..1318270(+) (addA) [Bacillus swezeyi strain EA208]
ATGGAAATGTCTAAGCCGAAGGGCAGCACATGGACTGATGATCAATGGAAGGCGATTGTCTCATCCGGCCGGGATATATT
AGTTGCCGCAGCGGCCGGTTCCGGAAAAACGGCCGTTCTAGTTGAACGGATCATCCGTAAAATTACCGAAAAGGAAAACC
CGATTGATGTTGACAGGCTTCTTGTCGTCACCTTTACGAATGCATCCGCGGCTGAAATGAAGCACCGGATCGGCGAAGCG
CTTGAAAAAGAGCTTGCCGAAAATCCCGGCTCCCTTCATTTGCGCAGACAGCTCGCCTTACTAAACAGAGCCAGCATATC
GACTCTGCACTCATTCTGTCTCCAGGTCGTTCGCAAATACTATTATCTGATCGACATTGATCCGGCTTTTCGGATCGCCG
ATCAGACAGAAGGCGAACTGCTTGGCGATGAGGTGCTTGATGACCTGTTTGAAGAAGAATATAAAAAGCAAGATCCGGCC
TTTTTCGAACTTGTGGACAGATATACGACAGACCGCCATGATCTTGATCTGCAATATCTTGTGAAGCGTGTCTACGAGTT
TTCAAGATCGCATCCTGATCCGGAAGGCTGGCTTGAAAAATTTGTTGACCTGTATGACGCCGCTCAAGATGAGAAAGTGG
AGTCGCTGCCGTTTTATTCTTATATCAAGGAAGATATTGCCCTCATCCTCGAAGGGGTGCGGGAAAAACTTGCGCGTGCG
CTTGAGCTGGCAAAACAGCCTGGAGGACCTGCACCGCGCGCCGAAAATTTCGTTGATGATTTAAAACAGATTGACAGGCT
GATTCAGCATCAGGATGATTTTTCGGCACTTTATGAGCTTGTTCCCGCTGTTTCATTTCAAAGGGCAAAGACCTGCAAGG
GAGACGAGTTTGAACCTGCCTTAATCGAAGAGTCGACAGAGCTTCGCAACAGTGCGAAAAAGCAATTGGAAAAGCTGAAA
AGCGATTATTTTACCCGGACCCCAGATCAGCATTTGGCGAGCCTGAAGGAAATGAAGCCTGTTATCCATACCCTTGTTCA
GCTTGTATTGGAGTATGGAAAGCGTTTCACAGCGGCCAAAAAGGAAAAGGCCATCGTTGATTTTTCTGATCTTGAACATG
ATTGCCTGGCCATTTTGACGAAGAAAAACGATGCCGGTGAAACCGGGCCGAGTGAAGCGGCAAAATATTACCGGCTCCAG
TTCCATGAAGTGCTTGTCGATGAGTATCAGGATACGAACCTTGTGCAAGAAGCGATTTTAAAGCTTGTCGCCAAAGATGA
GCCCGAGGGGAACCTGTTTATGGTGGGCGATGTCAAACAATCGATTTATCGGTTTCGGCTGGCTGAACCGCTTTTGTTTC
TCTCAAAATACAAACGATTTACAGAGGATGGCTCAGGCGCCGGCCAGAAAATCGATTTAAATAAAAATTTCAGAAGCCGT
TCAGATATATTAGACAGCACGAATTTCTTGTTTAAGCAGCTGATGGGGGAAAAGGTCGGAGAAGTTGACTATGATGAACA
AGCCGAATTAAAGCTCGGCGCATCATATCCTCCGAATGAAACGACGAAAACGGAGCTTTTGATCATTGAAAATCAAGGAG
ACGCAGCCGATGAAGAGGCCGAAGAGCTTGAAACGGTCCAGCTTGAAGCGAGAGCCATGGCGATGCAGATCCGCAAGCTG
ATATCCGAAAAGTTTCAAGTATATGACGGCAAAACGAAGACGACCCGCAATATCCAGTACCGCGACATCGTGATTCTGCT
GCGTTCGATGCCATGGGCGCCCCAGATTATGGATGAGTTCAAACAGCAGGGGATTCCCGTCTATGCGAATCTGTCTACAG
GCTATTTTGAAGCGACAGAAGTGTCCGTCACACTGTCGGTGTTAAAAGTCATTGACAATGCATACCAGGATATTCCGCTT
GCATCTGTTCTCAGGTCGCCTGTGGTCGGCCTTGATGAAAATGAGCTTTCCGTGATCAGGATTCAGGACAAGAAAGCGCC
GTTCTATGAAGCGATGAAAGCATATCTTGCTTCAGGTGACCGCGATGGCAGCCTTTATGAAAAGCTCCGCCGCTTTGAAG
GGCTTCTCAGAAAGTGGAGAGCGTATGCCAAAAACCATTCTGTGGCAGAGCTGATCTGGGAAATTTACCGTGATACGAAA
TACTTGGATTACGTCGGCGGAATGCCGGGCGGAAAACAGCGCCAGGCTAATTTGCGCGCGCTTTACGACCGGGCCCGATC
CTATGAAGCGACTTCTTTTCGCGGCCTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAACGCGGCGATGATCTCG
GAACGGCGAGAGCTCTGAGCGAACAGGAAGATGTCGTGCGGCTGATGACGATCCACTCAAGCAAGGGCCTTGAATTTCCC
GTCGTTTTCACAGCAGGCCTCGGCCGGAATTTTAATATGATGGATTTGAACAAGTCATATCTGCTTGATAAAGAACTCGG
CTTCGGAACAAAATTCATCCATCCGAAATGGCGCATCAGCTATCCGATGCTGCCGCTCGTCGCAATGAAGAAAAAAATGC
GCCGCGAGCTTTTGTCAGAGGAGCTGCGCGTCTTATATGTCGCTCTTACGCGGGCGAAGGAAAAGCTTTATTTGATCGGC
TCGGCAAAGGATAAGGAAAAGCTCTTGTCGAAATGGCGGACAACCGCAGCCCAGCCTGAGTGGCTGCTGCCGGACTTTGA
TCGCTTTCAGGCCAAATCGTATTTGGATTTTATCGGACCTGCATTGATGCGGCACAGGGATATGGCTGATGCAGGTGTGG
ATTCTCCGGATGACGAAATCCGGCAGCATCCATCACGTTTTCTCATCAGCTGGCTCTCGGGCGGAGAGCTCCAAGCTGAA
GACGCCAAACAAGCGGGGGAAGAAAAACACGAACGGCTGACTCAGATTAAAAAGGGGCTGCCGATCGATGCCGCGTTCGA
CTACCAGGAGGAGGTCGAGGAGCGGCTGAGCTGGTCATATCCTTATCAGGATGCGGCGAAAGTCCGGACGAAACAGTCCG
TCTCCGAAATCAAGAGACAAAAGGAATATGAGGATGAATACGGCGACCGTTCATTGATTCGTCCGGCAAATGAGGCACTG
CTGTTCAGACGGCCGTCCTTTATGATGACGAAGGGACTGACAGCCGCCGAACGGGGAACGGCGATGCATACCGTTATGCA
GCACATCCCGCTCACCCGGGCTCCGGAAAAAGACGAGCTCAGCCGCCTTCTTGACAGGCTTGTCGACAAAGAGCTTTTAA
CGGAAGAACAGCGGGCAGCAATTGAAGAAGATGATATTCTCGCATTCTTCGAAACAGATATAGGTGAAAAACTTCTCGGC
GCTCACCGGGTAGAGCGCGAAGTCCCCTTTAATATGACACTGCCGGCGGCAGAGGTTTATCCCAACCTGCAGACGGCGGA
TGAACCTGTATTGATTCAGGGGATTATCGACTGTTTATTTGAGACAGAGGACGGCTTTTATCTGCTTGATTACAAAACAG
ACCGAATCCAAGGAAAATACCGGAACGGCTTTGAGGGCGCCGAGCCGGTTTTAAGAAAACGCTATGAAACACAGATTCAT
CTTTATGCGCGCGCCGTTGAAACGATGCTCAAGATGCCGCTGAAAGGGCGTGCCCTGTACTTTTTTGACGGCGGACATGT
GCTTAAGTTTTAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1050-1222)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-881)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	73.924	100	0.74