Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WHU05_RS17960 Genome accession   NZ_CP148363
Coordinates   3659977..3660786 (-) Length   269 a.a.
NCBI ID   WP_001357652.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323HEC0355     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3654977..3665786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU05_RS17945 (WHU05_17925) gspD 3656434..3658494 (-) 2061 WP_000498844.1 type II secretion system secretin GspD -
  WHU05_RS17950 (WHU05_17930) gspC 3658524..3659483 (-) 960 WP_001357651.1 type II secretion system protein GspC -
  WHU05_RS17955 (WHU05_17935) gspS2 3659501..3659911 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  WHU05_RS17960 (WHU05_17940) pilD 3659977..3660786 (-) 810 WP_001357652.1 prepilin peptidase PppA Machinery gene
  WHU05_RS17965 (WHU05_17945) sslE 3660916..3665472 (-) 4557 WP_001357653.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29477.12 Da        Isoelectric Point: 8.3801

>NTDB_id=870738 WHU05_RS17960 WP_001357652.1 3659977..3660786(-) (pilD) [Escherichia coli strain Z1323HEC0355]
MFFDVFQQYPAVMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=870738 WHU05_RS17960 WP_001357652.1 3659977..3660786(-) (pilD) [Escherichia coli strain Z1323HEC0355]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGTGATGCCCGTCCTGGCTACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCATGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTTGAGTT
ATTGACAGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGACTGATTGCGGCATGGGCGCAGCAGAGTCCGTTAACGCTACAAGACGCAGTCACCGGCGTTCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTGGCTTTAATCGCATCATGCTGCGGTCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

42.066

100

0.424
  pilD Neisseria gonorrhoeae MS11

38.911

95.539

0.372
  pilD Acinetobacter nosocomialis M2

39.357

92.565

0.364
  pilD Acinetobacter baumannii D1279779

38.956

92.565

0.361