Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WHU04_RS14410 Genome accession   NZ_CP148357
Coordinates   2806586..2808019 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain Z1323HEC0393     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2801586..2813019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU04_RS14380 (WHU04_14350) yrbG 2802144..2803121 (+) 978 WP_000922877.1 calcium/sodium antiporter -
  WHU04_RS14385 (WHU04_14355) kdsD 2803135..2804121 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  WHU04_RS14390 (WHU04_14360) kdsC 2804142..2804708 (+) 567 WP_000030007.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  WHU04_RS14395 (WHU04_14365) lptC 2804705..2805280 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  WHU04_RS14400 (WHU04_14370) lptA 2805249..2805806 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  WHU04_RS14405 (WHU04_14375) lptB 2805813..2806538 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  WHU04_RS14410 (WHU04_14380) rpoN/rpoN1 2806586..2808019 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  WHU04_RS14415 (WHU04_14385) hpf 2808042..2808329 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  WHU04_RS14420 (WHU04_14390) ptsN 2808447..2808938 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WHU04_RS14425 (WHU04_14395) rapZ 2808984..2809838 (+) 855 WP_000243741.1 RNase adapter RapZ -
  WHU04_RS14430 (WHU04_14400) npr 2809835..2810107 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  WHU04_RS14435 (WHU04_14405) yrbL 2810320..2810952 (+) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  WHU04_RS14440 (WHU04_14410) mtgA 2810949..2811677 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  WHU04_RS14445 (WHU04_14415) elbB 2811674..2812327 (-) 654 WP_001295558.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=870641 WHU04_RS14410 WP_000809051.1 2806586..2808019(+) (rpoN/rpoN1) [Escherichia coli strain Z1323HEC0393]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=870641 WHU04_RS14410 WP_000809051.1 2806586..2808019(+) (rpoN/rpoN1) [Escherichia coli strain Z1323HEC0393]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465