Detailed information NTG0870425

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RAM73_RS14700
• Genome accession: NZ_CP132736
• Coordinates: 2981807..2982607 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain LZSFT39
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2976807..2987607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAM73_RS14665 (RAM73_14650)	gspH	2976963..2977478 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
RAM73_RS14670 (RAM73_14655)	gspI	2977475..2977840 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
RAM73_RS14675 (RAM73_14660)	gspJ	2977837..2978433 (+)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
RAM73_RS14680 (RAM73_14665)	gspK	2978430..2979416 (+)	987	WP_080831503.1	type II secretion system minor pseudopilin GspK	-
RAM73_RS14685 (RAM73_14670)	gspL	2979403..2980596 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
RAM73_RS14690 (RAM73_14675)	-	2980589..2981074 (+)	486	WP_004151943.1	type II secretion system protein M	-
RAM73_RS14695 (RAM73_14680)	-	2981064..2981810 (+)	747	WP_021441215.1	type II secretion system protein N	-
RAM73_RS14700 (RAM73_14685)	pilD	2981807..2982607 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
RAM73_RS14705 (RAM73_14690)	hrpB	2982655..2985084 (-)	2430	WP_251889568.1	ATP-dependent helicase HrpB	-
RAM73_RS14710 (RAM73_14695)	thpR	2985157..2985693 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
RAM73_RS14715 (RAM73_14700)	sfsA	2985693..2986409 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
RAM73_RS14720 (RAM73_14705)	dksA	2986572..2987027 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=870425 RAM73_RS14700 WP_004145898.1 2981807..2982607(+) (pilD) [Klebsiella pneumoniae strain LZSFT39]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=870425 RAM73_RS14700 WP_004145898.1 2981807..2982607(+) (pilD) [Klebsiella pneumoniae strain LZSFT39]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425