Detailed information NTG0869700

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RA221_RS21330
• Genome accession: NZ_CP132609
• Coordinates: 4367028..4367828 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 352310
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4362028..4372828

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RA221_RS21295 (RA221_21275)	gspH	4362184..4362699 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
RA221_RS21300 (RA221_21280)	gspI	4362696..4363061 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
RA221_RS21305 (RA221_21285)	gspJ	4363058..4363654 (+)	597	WP_009309266.1	type II secretion system minor pseudopilin GspJ	-
RA221_RS21310 (RA221_21290)	gspK	4363651..4364637 (+)	987	WP_077258397.1	type II secretion system minor pseudopilin GspK	-
RA221_RS21315 (RA221_21295)	gspL	4364624..4365817 (+)	1194	WP_048331607.1	type II secretion system protein GspL	-
RA221_RS21320 (RA221_21300)	-	4365810..4366295 (+)	486	WP_101529109.1	type II secretion system protein M	-
RA221_RS21325 (RA221_21305)	-	4366285..4367031 (+)	747	WP_087791831.1	type II secretion system protein N	-
RA221_RS21330 (RA221_21310)	pilD	4367028..4367828 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
RA221_RS21335 (RA221_21315)	hrpB	4367876..4370305 (-)	2430	WP_064153122.1	ATP-dependent helicase HrpB	-
RA221_RS21340 (RA221_21320)	thpR	4370378..4370914 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
RA221_RS21345 (RA221_21325)	sfsA	4370914..4371630 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
RA221_RS21350 (RA221_21330)	dksA	4371793..4372248 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=869700 RA221_RS21330 WP_004145898.1 4367028..4367828(+) (pilD) [Klebsiella pneumoniae strain 352310]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=869700 RA221_RS21330 WP_004145898.1 4367028..4367828(+) (pilD) [Klebsiella pneumoniae strain 352310]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425