Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   RAM43_RS00215 Genome accession   NZ_CP132394
Coordinates   46102..47451 (-) Length   449 a.a.
NCBI ID   WP_021758189.1    Uniprot ID   Q3YK46
Organism   Staphylococcus pseudintermedius strain CUVET18-689     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 37687..52732 46102..47451 within 0


Gene organization within MGE regions


Location: 37687..52732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM43_RS00175 (RAM43_00175) mecA 37687..39696 (-) 2010 WP_412549504.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  RAM43_RS00180 (RAM43_00180) - 39793..40779 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  RAM43_RS00185 (RAM43_00185) - 40769..41011 (+) 243 Protein_34 type I restriction enzyme endonuclease domain-containing protein -
  RAM43_RS00190 (RAM43_00190) - 41002..42612 (-) 1611 Protein_35 IS1182 family transposase -
  RAM43_RS00195 (RAM43_00195) - 42661..43167 (-) 507 WP_001092195.1 DUF1643 domain-containing protein -
  RAM43_RS00200 (RAM43_00200) - 43182..43493 (-) 312 WP_021758187.1 DUF960 domain-containing protein -
  RAM43_RS00205 (RAM43_00205) - 43580..43930 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  RAM43_RS00210 (RAM43_00210) ccrB 44452..46080 (-) 1629 WP_021758188.1 cassette chromosome recombinase CcrB Machinery gene
  RAM43_RS00215 (RAM43_00215) ccrA 46102..47451 (-) 1350 WP_021758189.1 cassette chromosome recombinase CcrA Machinery gene
  RAM43_RS00220 (RAM43_00220) cch1 47685..49478 (-) 1794 WP_021758190.1 cassette chromosome replicative helicase -
  RAM43_RS00225 (RAM43_00225) ssb 49478..49771 (-) 294 WP_021758191.1 cassette chromosome ssDNA-binding protein -
  RAM43_RS00230 (RAM43_00230) - 50006..51147 (+) 1142 WP_089535815.1 IS3 family transposase -
  RAM43_RS00235 (RAM43_00235) - 51218..52732 (-) 1515 WP_412549505.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52568.15 Da        Isoelectric Point: 9.9150

>NTDB_id=869045 RAM43_RS00215 WP_021758189.1 46102..47451(-) (ccrA) [Staphylococcus pseudintermedius strain CUVET18-689]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQVTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTVRKKNHILRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLHQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=869045 RAM43_RS00215 WP_021758189.1 46102..47451(-) (ccrA) [Staphylococcus pseudintermedius strain CUVET18-689]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAGTAACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATTAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTATGTGATTTCAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACATCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YK46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

98.218

100

0.982
  ccrA Staphylococcus aureus COL

74.61

100

0.746