Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RAM31_RS09145 Genome accession   NZ_CP132368
Coordinates   1938396..1939082 (-) Length   228 a.a.
NCBI ID   WP_001793467.1    Uniprot ID   A0A641AAY3
Organism   Staphylococcus aureus strain UNC_SA44     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1933396..1944082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM31_RS09125 (RAM31_09120) rpsT 1934310..1934561 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  RAM31_RS09130 (RAM31_09125) holA 1934606..1935580 (-) 975 WP_001282568.1 DNA polymerase III subunit delta -
  RAM31_RS09135 (RAM31_09130) comEC 1935637..1937838 (-) 2202 WP_001566670.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RAM31_RS09140 (RAM31_09135) - 1937843..1938304 (-) 462 WP_000439693.1 ComE operon protein 2 -
  RAM31_RS09145 (RAM31_09140) comEA 1938396..1939082 (-) 687 WP_001793467.1 ComEA family DNA-binding protein Machinery gene
  RAM31_RS09150 (RAM31_09145) - 1939122..1939838 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  RAM31_RS09155 (RAM31_09150) rsfS 1939841..1940194 (-) 354 WP_001088022.1 ribosome silencing factor -
  RAM31_RS09160 (RAM31_09155) yqeK 1940195..1940779 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RAM31_RS09165 (RAM31_09160) nadD 1940769..1941338 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RAM31_RS09170 (RAM31_09165) yhbY 1941341..1941631 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  RAM31_RS09175 (RAM31_09170) aroE 1941635..1942441 (-) 807 WP_000666748.1 shikimate dehydrogenase -
  RAM31_RS09180 (RAM31_09175) yqeH 1942455..1943555 (-) 1101 WP_306178500.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25786.50 Da        Isoelectric Point: 9.6753

>NTDB_id=868629 RAM31_RS09145 WP_001793467.1 1938396..1939082(-) (comEA) [Staphylococcus aureus strain UNC_SA44]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=868629 RAM31_RS09145 WP_001793467.1 1938396..1939082(-) (comEA) [Staphylococcus aureus strain UNC_SA44]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATAATG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641AAY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

100

100

1
  comEA Staphylococcus aureus N315

97.368

100

0.974