Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RAM31_RS08920 Genome accession   NZ_CP132368
Coordinates   1893420..1894394 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain UNC_SA44     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1888420..1899394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM31_RS08880 (RAM31_08875) gcvT 1888921..1890012 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  RAM31_RS08885 (RAM31_08880) - 1890171..1890695 (-) 525 WP_001015121.1 shikimate kinase -
  RAM31_RS08890 (RAM31_08885) - 1890685..1890831 (-) 147 WP_001789879.1 hypothetical protein -
  RAM31_RS08895 (RAM31_08890) comGF 1890928..1891425 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM31_RS08900 (RAM31_08895) comGE 1891343..1891642 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  RAM31_RS08905 (RAM31_08900) comGD 1891629..1892075 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM31_RS08910 (RAM31_08905) comGC 1892053..1892364 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM31_RS08915 (RAM31_08910) comGB 1892378..1893448 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM31_RS08920 (RAM31_08915) comGA 1893420..1894394 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM31_RS08925 (RAM31_08920) - 1894446..1895069 (-) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  RAM31_RS08930 (RAM31_08925) - 1895066..1895395 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM31_RS08935 (RAM31_08930) - 1895395..1896381 (-) 987 WP_000161314.1 ROK family glucokinase -
  RAM31_RS08940 (RAM31_08935) - 1896378..1896581 (-) 204 WP_000087561.1 YqgQ family protein -
  RAM31_RS08945 (RAM31_08940) - 1896562..1898025 (-) 1464 WP_001017865.1 rhomboid family intramembrane serine protease -
  RAM31_RS08950 (RAM31_08945) - 1898038..1898577 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  RAM31_RS08955 (RAM31_08950) rpmG 1898786..1898935 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=868626 RAM31_RS08920 WP_000697220.1 1893420..1894394(-) (comGA) [Staphylococcus aureus strain UNC_SA44]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=868626 RAM31_RS08920 WP_000697220.1 1893420..1894394(-) (comGA) [Staphylococcus aureus strain UNC_SA44]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAAAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1