Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   RAM28_RS08345 Genome accession   NZ_CP132362
Coordinates   1658668..1660869 (+) Length   733 a.a.
NCBI ID   WP_001557362.1    Uniprot ID   A0A6K1MRM3
Organism   Staphylococcus aureus strain UNC_SA55     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653668..1665869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM28_RS08305 (RAM28_08290) aroE 1654065..1654871 (+) 807 WP_000666757.1 shikimate dehydrogenase -
  RAM28_RS08310 (RAM28_08295) yhbY 1654875..1655165 (+) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  RAM28_RS08315 (RAM28_08300) nadD 1655168..1655737 (+) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RAM28_RS08320 (RAM28_08305) yqeK 1655727..1656311 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RAM28_RS08325 (RAM28_08310) rsfS 1656312..1656665 (+) 354 WP_001088020.1 ribosome silencing factor -
  RAM28_RS08330 (RAM28_08315) - 1656668..1657384 (+) 717 WP_000084830.1 class I SAM-dependent methyltransferase -
  RAM28_RS08335 (RAM28_08320) comEA 1657424..1658110 (+) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  RAM28_RS08340 (RAM28_08325) - 1658202..1658663 (+) 462 WP_000439693.1 ComE operon protein 2 -
  RAM28_RS08345 (RAM28_08330) comEC 1658668..1660869 (+) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RAM28_RS08350 (RAM28_08335) holA 1660926..1661900 (+) 975 WP_001282563.1 DNA polymerase III subunit delta -
  RAM28_RS08355 (RAM28_08340) rpsT 1661945..1662196 (-) 252 WP_001274017.1 30S ribosomal protein S20 -
  RAM28_RS08360 (RAM28_08345) lepA 1662542..1664365 (+) 1824 WP_000368338.1 translation elongation factor 4 -

Sequence

Protein


Download         Length: 733 a.a.        Molecular weight: 84998.05 Da        Isoelectric Point: 10.0028

>NTDB_id=868496 RAM28_RS08345 WP_001557362.1 1658668..1660869(+) (comEC) [Staphylococcus aureus strain UNC_SA55]
MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQ
VIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGIT
IIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQ
QLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFH
DWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSI
LYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKG
YSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLL
KKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
KVDSNSYGNASGL

Nucleotide


Download         Length: 2202 bp        

>NTDB_id=868496 RAM28_RS08345 WP_001557362.1 1658668..1660869(+) (comEC) [Staphylococcus aureus strain UNC_SA55]
TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTCTCTACATTTCTTTTCATTTT
ACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGT
ATTTACATTATTCACAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAATGAAATATATCCTTTCTTTTT
AACAGATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGAAA
ATAAGTTTGTAACTCTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATTATGGCATTGATTACTGGTGA
CGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGC
ATATAGCTGCAATTGTATTCTTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
ATCATTGTATTAACTTTATATGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCTATAATAATGACAACTCTTGT
ACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAACC
CACTAGTTGTTTATGATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTTATCGTTGCCATTCCAAACTT
TCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTA
TTTTATTCTTTATTACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
GACTGGTTACTAGACCTATTCACAAGAATTAAGCAATCACATTTTTCTGTTCCCAAGTTTAATGATTGGATATTTATAAT
ATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCTAAACGTAAATATATATTGGTTACGTTTTGGACTATAATTA
TTCTGACATTATTAATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGATGATACTAAACAACCTAGTTA
TTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATC
CACACAATGACCATATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTTATGGATGTAAGACAAGTTAG
TAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGA
TTATTACTATGATTACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACAAGTAGTTCTAAAGAATTTAT
AGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTA
AACGATTGCAAAGGATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(474-679)

Competence protein (Competence)

(183-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6K1MRM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Staphylococcus aureus N315

100

100

1
  comEC Staphylococcus aureus MW2

98.772

100

0.988