Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   Q8W18_RS11510 Genome accession   NZ_CP132058
Coordinates   2258607..2259677 (-) Length   356 a.a.
NCBI ID   WP_257474620.1    Uniprot ID   -
Organism   Bacillus pumilus strain F12-21     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2253607..2264677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q8W18_RS11460 (Q8W18_11460) sipW 2253700..2254272 (-) 573 WP_169056999.1 signal peptidase I SipW -
  Q8W18_RS11465 (Q8W18_11465) tapA 2254324..2254851 (-) 528 WP_306010869.1 amyloid fiber anchoring/assembly protein TapA -
  Q8W18_RS11470 (Q8W18_11470) - 2255151..2255429 (+) 279 WP_234034921.1 DUF3889 domain-containing protein -
  Q8W18_RS11475 (Q8W18_11475) - 2255463..2255657 (-) 195 WP_044140973.1 YqzE family protein -
  Q8W18_RS11480 (Q8W18_11480) comGG 2255717..2256100 (-) 384 WP_306010870.1 competence type IV pilus minor pilin ComGG -
  Q8W18_RS11485 (Q8W18_11485) comGF 2256097..2256627 (-) 531 WP_306010871.1 competence type IV pilus minor pilin ComGF -
  Q8W18_RS11490 (Q8W18_11490) comGE 2256536..2256853 (-) 318 WP_267903499.1 competence type IV pilus minor pilin ComGE -
  Q8W18_RS11495 (Q8W18_11495) comGD 2256837..2257283 (-) 447 WP_169056995.1 competence type IV pilus minor pilin ComGD -
  Q8W18_RS11500 (Q8W18_11500) comGC 2257276..2257569 (-) 294 WP_306010872.1 competence type IV pilus major pilin ComGC Machinery gene
  Q8W18_RS11505 (Q8W18_11505) comGB 2257586..2258626 (-) 1041 WP_306010873.1 competence type IV pilus assembly protein ComGB -
  Q8W18_RS11510 (Q8W18_11510) comGA 2258607..2259677 (-) 1071 WP_257474620.1 competence type IV pilus ATPase ComGA Machinery gene
  Q8W18_RS11515 (Q8W18_11515) - 2259937..2260527 (-) 591 WP_306010874.1 hypothetical protein -
  Q8W18_RS11520 (Q8W18_11520) - 2260925..2261797 (-) 873 WP_169056990.1 STAS domain-containing protein -
  Q8W18_RS11530 (Q8W18_11530) - 2262021..2262401 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  Q8W18_RS11535 (Q8W18_11535) - 2262615..2262857 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  Q8W18_RS11540 (Q8W18_11540) - 2262905..2263543 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  Q8W18_RS11545 (Q8W18_11545) - 2263701..2263874 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  Q8W18_RS11550 (Q8W18_11550) - 2263899..2264213 (-) 315 WP_306010875.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40334.72 Da        Isoelectric Point: 9.2484

>NTDB_id=867054 Q8W18_RS11510 WP_257474620.1 2258607..2259677(-) (comGA) [Bacillus pumilus strain F12-21]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=867054 Q8W18_RS11510 WP_257474620.1 2258607..2259677(-) (comGA) [Bacillus pumilus strain F12-21]
TTGTATGGGATTGAATATTTGGGACAAGAGCTACTAGAAGAAGCATGCCGTATGAGGGCATCTGATGTGCATATTGTCCC
AAAAGAAAAGGAAGCGTCTGTATCTTTCCGAGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCGTCCATGGATATCGGGGAAAAACGGAGACCGCAAAATGGGTCGCTAGCT
GTGATGCTGAGAAGAGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAAAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCTACTCGATTATTGTCGTTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGCAAAACAACGACCCTCTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATAATTACACTTGAAGATCCAGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTCCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGTTGAGTACACTGCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGTC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTCGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAGGAAGCAAGCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643