Detailed information NTG0867032

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: Q8W18_RS05605
• Genome accession: NZ_CP132058
• Coordinates: 1125401..1129099 (+)
• Length: 1232 a.a.
• NCBI ID: WP_306011963.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain F12-21
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1120401..1134099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q8W18_RS05590 (Q8W18_05590)	-	1120697..1121107 (+)	411	WP_180309273.1	DoxX family protein	-
Q8W18_RS05595 (Q8W18_05595)	-	1121303..1121749 (-)	447	WP_306011961.1	ferritin-like domain-containing protein	-
Q8W18_RS05600 (Q8W18_05600)	addB	1121914..1125423 (+)	3510	WP_306011962.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
Q8W18_RS05605 (Q8W18_05605)	addA	1125401..1129099 (+)	3699	WP_306011963.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
Q8W18_RS05610 (Q8W18_05610)	sbcD	1129126..1130301 (+)	1176	WP_058014359.1	exonuclease subunit SbcD	-
Q8W18_RS05615 (Q8W18_05615)	-	1130298..1133693 (+)	3396	WP_306011964.1	SMC family ATPase	-
Q8W18_RS05620 (Q8W18_05620)	-	1133713..1134012 (+)	300	WP_306011965.1	HNH endonuclease	-

Sequence

Protein

• Length: 1232 a.a.
• Molecular weight: 142247.28 Da
• Isoelectric Point: 5.0931

>NTDB_id=867032 Q8W18_RS05605 WP_306011963.1 1125401..1129099(+) (addA) [Bacillus pumilus strain F12-21]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHIRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDIDAQTKVEELPFYPYIKEDLTLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMRPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQNAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEVETSEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSNKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQFLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEEAGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3699 bp

>NTDB_id=867032 Q8W18_RS05605 WP_306011963.1 1125401..1129099(+) (addA) [Bacillus pumilus strain F12-21]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCGGGTAAAACAGCTGTTTTGGTTGAACGTCTGATTCGAAAAATGACTCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTTGTTGTAACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGAATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATAAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGTTTTCGTTTGGCCG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGACCGGTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCAAGATCTCATCCATCTCCTGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATATAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTACTCTCGTTCTCCGGAGCTGTCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACATCAATTTTAAAAGGCTCAAAACATGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAAAAAGCCACAGATGCACGTAATCAAGCGAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAGGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATTCAATTTGGTGAGCGTTTTGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGATTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGAATGCAGAAGGACATTTGATTGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTTGTTGATGAATATCAAGATACAAACCTTGTACAAGAAACCATTTTAAAGCTTGTATCTAAAGGAGA
ACATTCTGCTGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAACTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTTCACTTAG
ATCAGCAGGAAGTAGAAACCAGCGAAGAGCGTGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCACAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAATTGGCTCTGATTCGAACAAGTAA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTCCTTCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGG
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGGTTTGGAAGCAAGTACATCCATCCTGAACTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGAGTCCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
TCCTGATTTTGAACGGTATCAATCAAAAACATACTTAGATTTTATTGGACCTGCCCTCATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAGCAGGAGATGTTGTTCTTTCACATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCTGAGGAGCCTTATGATGAATCACGGATAGGACACTTGCTCGAATCTTTACAGCAGC
GAGACTTGCTAACAGATGAGCAGATCCAGTCAATTAATCAAGAAGGAATTGCATCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCTTTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
AGATACTGAGGGAGAGCCTGTACTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCATTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGATGGGAA
TTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1224)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.424	100	0.666