Detailed information NTG0867031

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: Q8W18_RS05600
• Genome accession: NZ_CP132058
• Coordinates: 1121914..1125423 (+)
• Length: 1169 a.a.
• NCBI ID: WP_306011962.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain F12-21
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1116914..1130423

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q8W18_RS05570 (Q8W18_05570)	lepB	1117432..1117944 (+)	513	WP_306011959.1	signal peptidase I	-
Q8W18_RS05575 (Q8W18_05575)	-	1117980..1119119 (-)	1140	WP_306011960.1	AbrB family transcriptional regulator	-
Q8W18_RS05580 (Q8W18_05580)	-	1119386..1119808 (+)	423	WP_058014365.1	BlaI/MecI/CopY family transcriptional regulator	-
Q8W18_RS05585 (Q8W18_05585)	-	1119801..1120640 (+)	840	WP_169056242.1	M56 family metallopeptidase	-
Q8W18_RS05590 (Q8W18_05590)	-	1120697..1121107 (+)	411	WP_180309273.1	DoxX family protein	-
Q8W18_RS05595 (Q8W18_05595)	-	1121303..1121749 (-)	447	WP_306011961.1	ferritin-like domain-containing protein	-
Q8W18_RS05600 (Q8W18_05600)	addB	1121914..1125423 (+)	3510	WP_306011962.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
Q8W18_RS05605 (Q8W18_05605)	addA	1125401..1129099 (+)	3699	WP_306011963.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
Q8W18_RS05610 (Q8W18_05610)	sbcD	1129126..1130301 (+)	1176	WP_058014359.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1169 a.a.
• Molecular weight: 134913.77 Da
• Isoelectric Point: 5.2323

>NTDB_id=867031 Q8W18_RS05600 WP_306011962.1 1121914..1125423(+) (addB) [Bacillus pumilus strain F12-21]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKAMAEFKRYCMLPEDIEKISVESMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISETIFERNHRHLYTPDIAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKELRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IVPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLDEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDNEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIYKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide

• Length: 3510 bp

>NTDB_id=867031 Q8W18_RS05600 WP_306011962.1 1121914..1125423(+) (addB) [Bacillus pumilus strain F12-21]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCATTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCAAAAGTTTTTAGTTTTACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAGCGATGGCTGAATTCAAAAGATACT
GTATGCTGCCTGAGGATATTGAGAAAATTTCAGTGGAGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCATTCAGAAGACTATTT
GACTCTTCTTGCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATTTATATCGATGGTTTCTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTCCATGCTGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCGAA
AGAGTGCGGAGCTGACATTTCTGAAACCATTTTCGAAAGAAATCATCGGCACCTTTATACACCAGATATTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTACAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGAACTTAGACTCCGAGATATCTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGACATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAATATCATCCGCTTATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACAAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAATGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATCGAAATGGAACAAATGCTAAATGATGTCAAAGAATGG
ATCGTGCCCCCGCTTTACCAGTTGCAGAAAAGACTTAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGACAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGATGAAG
CAATGCAGCACGGGCAAGTATGGGATGCTGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGATGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGATCA
AGTATTTATCGGAAGTATGGATTTATCCAGGATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAACGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAAGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTTTATTGCCTTCAACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACAATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACTGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CTTTAAAGCCAAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAAAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTTAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGACTTGAGTTAGGTTTCGGGGGGCAAGGACCTTTGCCGCCATTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAACTTGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCAATTCCGCTTGCAGAAGATGAAATTGAGCAGGAAATATACAAGAAGTTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCTGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTACTTACCTCTGA
AAAGGACGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	66.15	99.316	0.657