Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q7574_RS13905 Genome accession   NZ_CP131867
Coordinates   2924841..2925959 (+) Length   372 a.a.
NCBI ID   WP_005070855.1    Uniprot ID   A0A013SVN8
Organism   Acinetobacter pittii strain 2012N08-034     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2919841..2930959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7574_RS13895 (Q7574_13955) yggS 2922965..2923657 (-) 693 WP_151283388.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q7574_RS13900 (Q7574_13960) pilT 2923784..2924821 (+) 1038 WP_002121749.1 type IV pilus twitching motility protein PilT Machinery gene
  Q7574_RS13905 (Q7574_13965) pilU 2924841..2925959 (+) 1119 WP_005070855.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q7574_RS13910 (Q7574_13970) fur 2926029..2926466 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  Q7574_RS13915 (Q7574_13975) omlA 2926578..2926976 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  Q7574_RS13920 (Q7574_13980) - 2927041..2927355 (-) 315 WP_014205982.1 RnfH family protein -
  Q7574_RS13925 (Q7574_13985) - 2927355..2928422 (-) 1068 WP_151283389.1 hypothetical protein -
  Q7574_RS13930 (Q7574_13990) - 2928576..2929031 (-) 456 WP_000782976.1 bacteriohemerythrin -
  Q7574_RS13935 (Q7574_13995) - 2929283..2930131 (-) 849 WP_151283391.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41931.15 Da        Isoelectric Point: 6.6404

>NTDB_id=865635 Q7574_RS13905 WP_005070855.1 2924841..2925959(+) (pilU) [Acinetobacter pittii strain 2012N08-034]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=865635 Q7574_RS13905 WP_005070855.1 2924841..2925959(+) (pilU) [Acinetobacter pittii strain 2012N08-034]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTAAGGCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTGCTTAAAGATTTATCGATGACTAAACGCGGTATCATTATTTTTGTAGGTGCAACGG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCCATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAGAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCCGCAATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(33-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A013SVN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.731

0.685
  pilU Vibrio cholerae strain A1552

53.784

99.462

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.934

97.849

0.401
  pilT Legionella pneumophila strain Lp02

39.941

90.86

0.363
  pilT Legionella pneumophila strain ERS1305867

39.941

90.86

0.363