Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   Q7609_RS03720 Genome accession   NZ_CP131725
Coordinates   723533..724072 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain 2018S05-172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 718533..729072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7609_RS03690 (Q7609_03690) fdx 718936..719277 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  Q7609_RS03695 (Q7609_03695) iscX 719277..719471 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  Q7609_RS03700 (Q7609_03700) - 719522..720136 (-) 615 WP_005654851.1 YfgM family protein -
  Q7609_RS03705 (Q7609_03705) hisS 720154..721425 (-) 1272 WP_005654849.1 histidine--tRNA ligase -
  Q7609_RS03710 (Q7609_03710) ispG 721435..722541 (-) 1107 WP_005654847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q7609_RS03715 (Q7609_03715) - 722550..723461 (-) 912 WP_005654845.1 RodZ family helix-turn-helix domain-containing protein -
  Q7609_RS03720 (Q7609_03720) pilF/pilF2 723533..724072 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q7609_RS03725 (Q7609_03725) - 724173..725324 (-) 1152 WP_005654842.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  Q7609_RS03730 (Q7609_03730) - 725571..726449 (+) 879 WP_005654840.1 serine hydrolase -
  Q7609_RS03735 (Q7609_03735) - 726815..727696 (+) 882 WP_005654837.1 metal ABC transporter substrate-binding protein -
  Q7609_RS03740 (Q7609_03740) - 727696..728616 (+) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=864867 Q7609_RS03720 WP_005654843.1 723533..724072(-) (pilF/pilF2) [Haemophilus influenzae strain 2018S05-172]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=864867 Q7609_RS03720 WP_005654843.1 723533..724072(-) (pilF/pilF2) [Haemophilus influenzae strain 2018S05-172]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447