Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   Q7612_RS03830 Genome accession   NZ_CP131722
Coordinates   742991..743530 (-) Length   179 a.a.
NCBI ID   WP_075913234.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2018C09-260     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737991..748530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7612_RS03800 (Q7612_03790) fdx 738394..738735 (+) 342 WP_342123033.1 ISC system 2Fe-2S type ferredoxin -
  Q7612_RS03805 (Q7612_03795) iscX 738735..738929 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  Q7612_RS03810 (Q7612_03800) - 738980..739594 (-) 615 WP_112109782.1 YfgM family protein -
  Q7612_RS03815 (Q7612_03805) hisS 739612..740883 (-) 1272 WP_342123034.1 histidine--tRNA ligase -
  Q7612_RS03820 (Q7612_03810) ispG 740893..741999 (-) 1107 WP_005656366.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q7612_RS03825 (Q7612_03815) - 742008..742919 (-) 912 WP_112119363.1 RodZ family helix-turn-helix domain-containing protein -
  Q7612_RS03830 (Q7612_03820) pilF/pilF2 742991..743530 (-) 540 WP_075913234.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q7612_RS03835 (Q7612_03825) - 743631..744782 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  Q7612_RS03840 (Q7612_03830) - 745029..745907 (+) 879 WP_112087529.1 serine hydrolase -
  Q7612_RS03845 (Q7612_03835) - 746273..747154 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  Q7612_RS03850 (Q7612_03840) - 747154..748074 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20568.22 Da        Isoelectric Point: 8.6216

>NTDB_id=864757 Q7612_RS03830 WP_075913234.1 742991..743530(-) (pilF/pilF2) [Haemophilus influenzae strain 2018C09-260]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=864757 Q7612_RS03830 WP_075913234.1 742991..743530(-) (pilF/pilF2) [Haemophilus influenzae strain 2018C09-260]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

99.441

100

0.994
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447