Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   Q7616_RS03730 Genome accession   NZ_CP131717
Coordinates   724544..725083 (-) Length   179 a.a.
NCBI ID   WP_075913234.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2016N21-284     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 719544..730083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7616_RS03700 (Q7616_03705) fdx 719947..720288 (+) 342 WP_118806694.1 ISC system 2Fe-2S type ferredoxin -
  Q7616_RS03705 (Q7616_03710) iscX 720288..720482 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  Q7616_RS03710 (Q7616_03715) - 720533..721147 (-) 615 WP_005693794.1 YfgM family protein -
  Q7616_RS03715 (Q7616_03720) hisS 721165..722436 (-) 1272 WP_116962329.1 histidine--tRNA ligase -
  Q7616_RS03720 (Q7616_03725) ispG 722446..723552 (-) 1107 WP_005656366.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Q7616_RS03725 (Q7616_03730) - 723561..724472 (-) 912 WP_112119363.1 RodZ family helix-turn-helix domain-containing protein -
  Q7616_RS03730 (Q7616_03735) pilF/pilF2 724544..725083 (-) 540 WP_075913234.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Q7616_RS03735 (Q7616_03740) - 725184..726335 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  Q7616_RS03740 (Q7616_03745) - 726582..727460 (+) 879 WP_112087529.1 serine hydrolase -
  Q7616_RS03745 (Q7616_03750) - 727826..728707 (+) 882 WP_116966159.1 metal ABC transporter substrate-binding protein -
  Q7616_RS03750 (Q7616_03755) - 728707..729627 (+) 921 WP_116967415.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20568.22 Da        Isoelectric Point: 8.6216

>NTDB_id=864613 Q7616_RS03730 WP_075913234.1 724544..725083(-) (pilF/pilF2) [Haemophilus influenzae strain 2016N21-284]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=864613 Q7616_RS03730 WP_075913234.1 724544..725083(-) (pilF/pilF2) [Haemophilus influenzae strain 2016N21-284]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

99.441

100

0.994
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447