Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   WHO32_RS06385 Genome accession   NZ_CP148112
Coordinates   1230852..1232681 (+) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis isolate FELIX_MS504     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1225852..1237681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO32_RS06350 yjbC 1226524..1227102 (+) 579 WP_003244921.1 GNAT family N-acetyltransferase -
  WHO32_RS06355 spx 1227283..1227678 (+) 396 WP_003245483.1 transcriptional regulator Spx -
  WHO32_RS06360 yjbE 1227721..1228377 (-) 657 WP_003232944.1 TerC family protein -
  WHO32_RS06365 - 1228547..1228687 (+) 141 WP_119122854.1 hypothetical protein -
  WHO32_RS06370 mecA 1228654..1229310 (+) 657 WP_003245194.1 adaptor protein MecA Regulator
  WHO32_RS06375 - 1229305..1229427 (-) 123 WP_003245684.1 hypothetical protein -
  WHO32_RS06380 coiA 1229471..1230622 (+) 1152 WP_003245839.1 competence protein CoiA Machinery gene
  WHO32_RS06385 pepF 1230852..1232681 (+) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  WHO32_RS06390 - 1232719..1232886 (-) 168 WP_003244944.1 hypothetical protein -
  WHO32_RS06395 spxH 1233200..1234099 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  WHO32_RS06400 yjbI 1234096..1234494 (-) 399 WP_003232928.1 group 2 truncated hemoglobin YjbI -
  WHO32_RS06405 cwlQ 1234749..1235483 (-) 735 WP_003245645.1 bifunctional muramidase/murein lytic transglycosylase -
  WHO32_RS06410 yjbK 1235498..1236070 (-) 573 WP_003232924.1 CYTH domain-containing protein -
  WHO32_RS06415 - 1236195..1236563 (+) 369 WP_003232922.1 hypothetical protein -
  WHO32_RS06420 yjbM 1236592..1237227 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=864596 WHO32_RS06385 WP_003245178.1 1230852..1232681(+) (pepF) [Bacillus subtilis isolate FELIX_MS504]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=864596 WHO32_RS06385 WP_003245178.1 1230852..1232681(+) (pepF) [Bacillus subtilis isolate FELIX_MS504]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTAGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTGAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCATCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCCACGACG
CTAAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTCGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATTTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATTCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GTTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476