Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WHL49_RS12785 Genome accession   NZ_CP148103
Coordinates   2493757..2494167 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis isolate FELIX_MS361     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2488757..2499167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHL49_RS12755 - 2488844..2488984 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  WHL49_RS12760 yqeF 2489290..2490021 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  WHL49_RS12765 cwlH 2490273..2491025 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  WHL49_RS12770 yqeD 2491212..2491838 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  WHL49_RS12775 gnd 2491857..2492750 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  WHL49_RS12780 yqeB 2493002..2493724 (+) 723 WP_010886572.1 hypothetical protein -
  WHL49_RS12785 nucA/comI 2493757..2494167 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  WHL49_RS12790 - 2494363..2494710 (+) 348 Protein_2469 sigma-70 family RNA polymerase sigma factor -
  WHL49_RS12795 spoIVCA 2494741..2496201 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  WHL49_RS12800 - 2496159..2496337 (-) 179 Protein_2471 hypothetical protein -
  WHL49_RS12805 arsC 2496692..2497111 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  WHL49_RS12810 acr3 2497123..2498163 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  WHL49_RS12815 yqcK 2498186..2498626 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  WHL49_RS12820 arsR 2498687..2499004 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=864085 WHL49_RS12785 WP_009967785.1 2493757..2494167(-) (nucA/comI) [Bacillus subtilis subsp. subtilis isolate FELIX_MS361]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=864085 WHL49_RS12785 WP_009967785.1 2493757..2494167(-) (nucA/comI) [Bacillus subtilis subsp. subtilis isolate FELIX_MS361]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529